LOCUS       sequence-138060-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  promoterless Tol2 vector with 6xmyc tag upstream of and in frame 
            with attR1/attR2 cassette and late SV40 polyadenylation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 252 pTol2-MTDest
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..46
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     30..46
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(80..91,92..279)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 agctggagct ccaccgcggt ggcggccgct ctagaactag tggatctgct gggcttgctg
       61 aaggtagggg gtcaagaacc agaggtgtaa agtacttgag taattttact tgattactgt
      121 acttaagtat tatttttggg gatttttact ttacttgagt acaattaaaa atcaatactt
      181 ttacttttac ttaattacat ttttttagaa aaaaaagtac tttttactcc ttacaatttt
      241 atttacagtc aaaaagtact tattttttgg agatcacttc attctatttt cccttgctat
      301 taccaaacca attgaattgc gctgatgccc agtttaattt aaatgttatt tattctgcct
      361 atgaaaatcg ttttcacatt atatgaaatt ggtcagacat gttcattggt cctttggaag
      421 tgacgtcatg tcacatctat taccacaatg cacagcacct tgacctggaa attagggaaa
      481 ttataacagt caatcagtgg aagaaaatgg aggaagtatg tgattcatca gcagctgcga
      541 gcagcacagt ccaaaatcag ccacaggatc aagagcaccc gtggccgtat cttcgcgaat
      601 tcttttcttt aagtggtgta aataaagatt cattcaagat gaaatgtgtc ctctgtctcc
      661 cgcttaataa
//
