LOCUS       sequence-138093-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express YFP-NLS under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  TITLE     plasmid for first generation adenovirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAdx-CMV-YFP
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..140)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    150..231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     175..194
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(286..306)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(310..330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(334..354)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(379..630)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(421..442)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctacataaga cccccacctt atatattctt tcccaccctt aaccacgccc agatcttcga
       61 gtcgatgcca tttcattacc tctttctccg cacccgacat agatacgcgc cacgcgtgca
      121 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      181 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tctagtcact
      241 tacttatcga tagaattcgc tcagttatct agatccggtg gagcctacct ttctcttctt
      301 ttttggatct acctttctct tcttttttgg atctaccttt ctcttctttt ttggatcagc
      361 tcgagatctg agtccggact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      421 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      481 ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      541 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      601 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
      661 g
//
