LOCUS       sequence-138095-       34300 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Express YFP-NLS under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 34300)
  TITLE     plasmid for first generation adenovirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAdx-CMV-YFP
FEATURES             Location/Qualifiers
     source          1..34300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        95..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(646..648,649..651,652..1362)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(691..712)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(952..971)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1299..1320
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1387..1407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1411..1431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1435..1455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1510..1591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1547..1566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1601..1620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(5210..5275,5276..5313)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(12407..12418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      19890..19899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   30859..30961
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(31040..31059)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(31041..31059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(31066..31083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(31066..31082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(31075..31097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(31223..31678)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(31360..31381)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     31572..31591
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        31757..31861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(31862..31930,31931..32722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(32080..32099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      32893..33481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     33382..33401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     33635..33652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    33769..33790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(33805..33810,33811..33828,33829..33835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    33843..33859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     33848..33870
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     33867..33883
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     33867..33883
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        33901..33919
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     33901..33918
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   33951..34053
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     34141..34291
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 cgtaactata acggtcctaa ggtagcgacg cgcctagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctggtttagt gaaccgtcag atccgctagc aagcttggat ccaccatggt gagcaagggc
      661 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      721 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      781 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      841 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      901 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      961 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1021 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1081 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1141 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1201 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1261 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1321 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga
     1381 gctgatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa
     1441 aagaagagaa aggtaggctc caccggatct agataactga gcgaattcta tcgataagta
     1501 agtgactaga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1621 tgcacgcgtg gcgcgtatct atgtcgggtg cggagaaaga ggtaatgaaa tggcatcgac
     1681 tcgaagatct gggcgtggtt aagggtggga aagaatatat aaggtggggg tcttatgtag
     1741 ttttgtatct gttttgcagc agccgccgcc gccatgagca ccaactcgtt tgatggaagc
     1801 attgtgagct catatttgac aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg
     1861 atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa actctactac cttgacctac
     1921 gagaccgtgt ctggaacgcc gttggagact gcagcctccg ccgccgcttc agccgctgca
     1981 gccaccgccc gcgggattgt gactgacttt gctttcctga gcccgcttgc aagcagtgca
     2041 gcttcccgtt catccgcccg cgatgacaag ttgacggctc ttttggcaca attggattct
     2101 ttgacccggg aacttaatgt cgtttctcag cagctgttgg atctgcgcca gcaggtttct
     2161 gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca taaataaaaa accagactct
     2221 gtttggattt ggatcaagca agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg
     2281 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg
     2341 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag
     2401 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag
     2461 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc
     2521 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga
     2581 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc
     2641 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc
     2701 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg
     2761 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg
     2821 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag
     2881 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta
     2941 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc
     3001 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc
     3061 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccggctgc
     3121 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta
     3181 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc
     3241 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta
     3301 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc
     3361 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt
     3421 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg
     3481 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca
     3541 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt
     3601 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg
     3661 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt
     3721 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc
     3781 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt
     3841 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct
     3901 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg
     3961 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc
     4021 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca
     4081 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt
     4141 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg
     4201 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt
     4261 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg
     4321 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt
     4381 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg
     4441 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg
     4501 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg
     4561 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa
     4621 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc
     4681 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg
     4741 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta
     4801 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc
     4861 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg
     4921 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca
     4981 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg
     5041 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg
     5101 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg
     5161 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt
     5221 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc
     5281 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
     5341 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct
     5401 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa
     5461 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc
     5521 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg
     5581 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg
     5641 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc
     5701 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag
     5761 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa
     5821 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca
     5881 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg
     5941 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg
     6001 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga
     6061 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga
     6121 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc
     6181 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact
     6241 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct
     6301 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc
     6361 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct
     6421 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg
     6481 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga
     6541 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca
     6601 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga
     6661 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg
     6721 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg
     6781 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg
     6841 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc
     6901 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgaacc tgaaagagag
     6961 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc
     7021 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag
     7081 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag
     7141 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc
     7201 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac
     7261 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac
     7321 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag
     7381 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga
     7441 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg
     7501 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc
     7561 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg
     7621 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac
     7681 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg
     7741 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg
     7801 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa
     7861 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg
     7921 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa
     7981 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg
     8041 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt
     8101 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc
     8161 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc
     8221 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac
     8281 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc
     8341 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca
     8401 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct
     8461 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg
     8521 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc
     8581 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc
     8641 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa
     8701 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagcg
     8761 tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc tggtggataa attcgcaagg
     8821 gtatcatggc ggacgaccgg ggttcgagcc ccgtatccgg ccgtccgccg tgatccatgc
     8881 ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag acaacggggg agtgctcctt
     8941 ttggcttcct tccaggcgcg gcggctgctg cgctagcttt tttggccact ggccgcgcgc
     9001 agcgtaagcg gttaggctgg aaagcgaaag cattaagtgg ctcgctccct gtagccggag
     9061 ggttattttc caagggttga gtcgcgggac ccccggttcg agtctcggac cggccggact
     9121 gcggcgaacg ggggtttgcc tccccgtcat gcaagacccc gcttgcaaat tcctccggaa
     9181 acagggacga gccccttttt tgcttttccc agatgcatcc ggtgctgcgg cagatgcgcc
     9241 cccctcctca gcagcggcaa gagcaagagc agcggcagac atgcagggca ccctcccctc
     9301 ctcctaccgc gtcaggaggg gcgacatccg cggttgacgc ggcagcagat ggtgattacg
     9361 aacccccgcg gcgccgggcc cggcactacc tggacttgga ggagggcgag ggcctggcgc
     9421 ggctaggagc gccctctcct gagcggcacc caagggtgca gctgaagcgt gatacgcgtg
     9481 aggcgtacgt gccgcggcag aacctgtttc gcgaccgcga gggagaggag cccgaggaga
     9541 tgcgggatcg aaagttccac gcagggcgcg agctgcggca tggcctgaat cgcgagcggt
     9601 tgctgcgcga ggaggacttt gagcccgacg cgcgaaccgg gattagtccc gcgcgcgcac
     9661 acgtggcggc cgccgacctg gtaaccgcat acgagcagac ggtgaaccag gagattaact
     9721 ttcaaaaaag ctttaacaac cacgtgcgta cgcttgtggc gcgcgaggag gtggctatag
     9781 gactgatgca tctgtgggac tttgtaagcg cgctggagca aaacccaaat agcaagccgc
     9841 tcatggcgca gctgttcctt atagtgcagc acagcaggga caacgaggca ttcagggatg
     9901 cgctgctaaa catagtagag cccgagggcc gctggctgct cgatttgata aacatcctgc
     9961 agagcatagt ggtgcaggag cgcagcttga gcctggctga caaggtggcc gccatcaact
    10021 attccatgct tagcctgggc aagttttacg cccgcaagat ataccatacc ccttacgttc
    10081 ccatagacaa ggaggtaaag atcgaggggt tctacatgcg catggcgctg aaggtgctta
    10141 ccttgagcga cgacctgggc gtttatcgca acgagcgcat ccacaaggcc gtgagcgtga
    10201 gccggcggcg cgagctcagc gaccgcgagc tgatgcacag cctgcaaagg gccctggctg
    10261 gcacgggcag cggcgataga gaggccgagt cctactttga cgcgggcgct gacctgcgct
    10321 gggccccaag ccgacgcgcc ctggaggcag ctggggccgg acctgggctg gcggtggcac
    10381 ccgcgcgcgc tggcaacgtc ggcggcgtgg aggaatatga cgaggacgat gagtacgagc
    10441 cagaggacgg cgagtactaa gcggtgatgt ttctgatcag atgatgcaag acgcaacgga
    10501 cccggcggtg cgggcggcgc tgcagagcca gccgtccggc cttaactcca cggacgactg
    10561 gcgccaggtc atggaccgca tcatgtcgct gactgcgcgc aatcctgacg cgttccggca
    10621 gcagccgcag gccaaccggc tctccgcaat tctggaagcg gtggtcccgg cgcgcgcaaa
    10681 ccccacgcac gagaaggtgc tggcgatcgt aaacgcgctg gccgaaaaca gggccatccg
    10741 gcccgacgag gccggcctgg tctacgacgc gctgcttcag cgcgtggctc gttacaacag
    10801 cggcaacgtg cagaccaacc tggaccggct ggtgggggat gtgcgcgagg ccgtggcgca
    10861 gcgtgagcgc gcgcagcagc agggcaacct gggctccatg gttgcactaa acgccttcct
    10921 gagtacacag cccgccaacg tgccgcgggg acaggaggac tacaccaact ttgtgagcgc
    10981 actgcggcta atggtgactg agacaccgca aagtgaggtg taccagtctg ggccagacta
    11041 ttttttccag accagtagac aaggcctgca gaccgtaaac ctgagccagg ctttcaaaaa
    11101 cttgcagggg ctgtgggggg tgcgggctcc cacaggcgac cgcgcgaccg tgtctagctt
    11161 gctgacgccc aactcgcgcc tgttgctgct gctaatagcg cccttcacgg acagtggcag
    11221 cgtgtcccgg gacacatacc taggtcactt gctgacactg taccgcgagg ccataggtca
    11281 ggcgcatgtg gacgagcata ctttccagga gattacaagt gtcagccgcg cgctggggca
    11341 ggaggacacg ggcagcctgg aggcaaccct aaactacctg ctgaccaacc ggcggcagaa
    11401 gatcccctcg ttgcacagtt taaacagcga ggaggagcgc attttgcgct acgtgcagca
    11461 gagcgtgagc cttaacctga tgcgcgacgg ggtaacgccc agcgtggcgc tggacatgac
    11521 cgcgcgcaac atggaaccgg gcatgtatgc ctcaaaccgg ccgtttatca accgcctaat
    11581 ggactacttg catcgcgcgg ccgccgtgaa ccccgagtat ttcaccaatg ccatcttgaa
    11641 cccgcactgg ctaccgcccc ctggtttcta caccggggga ttcgaggtgc ccgagggtaa
    11701 cgatggattc ctctgggacg acatagacga cagcgtgttt tccccgcaac cgcagaccct
    11761 gctagagttg caacagcgcg agcaggcaga ggcggcgctg cgaaaggaaa gcttccgcag
    11821 gccaagcagc ttgtccgatc taggcgctgc ggccccgcgg tcagatgcta gtagcccatt
    11881 tccaagcttg atagggtctc ttaccagcac tcgcaccacc cgcccgcgcc tgctgggcga
    11941 ggaggagtac ctaaacaact cgctgctgca gccgcagcgc gaaaaaaacc tgcctccggc
    12001 atttcccaac aacgggatag agagcctagt ggacaagatg agtagatgga agacgtacgc
    12061 gcaggagcac agggacgtgc caggcccgcg cccgcccacc cgtcgtcaaa ggcacgaccg
    12121 tcagcggggt ctggtgtggg aggacgatga ctcggcagac gacagcagcg tcctggattt
    12181 gggagggagt ggcaacccgt ttgcgcacct tcgccccagg ctggggagaa tgttttaaaa
    12241 aaaaaaaaag catgatgcaa aataaaaaac tcaccaaggc catggcaccg agcgttggtt
    12301 ttcttgtatt ccccttagta tgcggcgcgc ggcgatgtat gaggaaggtc ctcctccctc
    12361 ctacgagagt gtggtgagcg cggcgccagt ggcggcggcg ctgggttctc ccttcgatgc
    12421 tcccctggac ccgccgtttg tgcctccgcg gtacctgcgg cctaccgggg ggagaaacag
    12481 catccgttac tctgagttgg cacccctatt cgacaccacc cgtgtgtacc tggtggacaa
    12541 caagtcaacg gatgtggcat ccctgaacta ccagaacgac cacagcaact ttctgaccac
    12601 ggtcattcaa aacaatgact acagcccggg ggaggcaagc acacagacca tcaatcttga
    12661 cgaccggtcg cactggggcg gcgacctgaa aaccatcctg cataccaaca tgccaaatgt
    12721 gaacgagttc atgtttacca ataagtttaa ggcgcgggtg atggtgtcgc gcttgcctac
    12781 taaggacaat caggtggagc tgaaatacga gtgggtggag ttcacgctgc ccgagggcaa
    12841 ctactccgag accatgacca tagaccttat gaacaacgcg atcgtggagc actacttgaa
    12901 agtgggcaga cagaacgggg ttctggaaag cgacatcggg gtaaagtttg acacccgcaa
    12961 cttcagactg gggtttgacc ccgtcactgg tcttgtcatg cctggggtat atacaaacga
    13021 agccttccat ccagacatca ttttgctgcc aggatgcggg gtggacttca cccacagccg
    13081 cctgagcaac ttgttgggca tccgcaagcg gcaacccttc caggagggct ttaggatcac
    13141 ctacgatgat ctggagggtg gtaacattcc cgcactgttg gatgtggacg cctaccaggc
    13201 gagcttgaaa gatgacaccg aacagggcgg gggtggcgca ggcggcagca acagcagtgg
    13261 cagcggcgcg gaagagaact ccaacgcggc agccgcggca atgcagccgg tggaggacat
    13321 gaacgatcat gccattcgcg gcgacacctt tgccacacgg gctgaggaga agcgcgctga
    13381 ggccgaagca gcggccgaag ctgccgcccc cgctgcgcaa cccgaggtcg agaagcctca
    13441 gaagaaaccg gtgatcaaac ccctgacaga ggacagcaag aaacgcagtt acaacctaat
    13501 aagcaatgac agcaccttca cccagtaccg cagctggtac cttgcataca actacggcga
    13561 ccctcagacc ggaatccgct catggaccct gctttgcact cctgacgtaa cctgcggctc
    13621 ggagcaggtc tactggtcgt tgccagacat gatgcaagac cccgtgacct tccgctccac
    13681 gcgccagatc agcaactttc cggtggtggg cgccgagctg ttgcccgtgc actccaagag
    13741 cttctacaac gaccaggccg tctactccca actcatccgc cagtttacct ctctgaccca
    13801 cgtgttcaat cgctttcccg agaaccagat tttggcgcgc ccgccagccc ccaccatcac
    13861 caccgtcagt gaaaacgttc ctgctctcac agatcacggg acgctaccgc tgcgcaacag
    13921 catcggagga gtccagcgag tgaccattac tgacgccaga cgccgcacct gcccctacgt
    13981 ttacaaggcc ctgggcatag tctcgccgcg cgtcctatcg agccgcactt tttgagcaag
    14041 catgtccatc cttatatcgc ccagcaataa cacaggctgg ggcctgcgct tcccaagcaa
    14101 gatgtttggc ggggccaaga agcgctccga ccaacaccca gtgcgcgtgc gcgggcacta
    14161 ccgcgcgccc tggggcgcgc acaaacgcgg ccgcactggg cgcaccaccg tcgatgacgc
    14221 catcgacgcg gtggtggagg aggcgcgcaa ctacacgccc acgccgccac cagtgtccac
    14281 agtggacgcg gccattcaga ccgtggtgcg cggagcccgg cgctatgcta aaatgaagag
    14341 acggcggagg cgcgtagcac gtcgccaccg ccgccgaccc ggcactgccg cccaacgcgc
    14401 ggcggcggcc ctgcttaacc gcgcacgtcg caccggccga cgggcggcca tgcgggccgc
    14461 tcgaaggctg gccgcgggta ttgtcactgt gccccccagg tccaggcgac gagcggccgc
    14521 cgcagcagcc gcggccatta gtgctatgac tcagggtcgc aggggcaacg tgtattgggt
    14581 gcgcgactcg gttagcggcc tgcgcgtgcc cgtgcgcacc cgccccccgc gcaactagat
    14641 tgcaagaaaa aactacttag actcgtactg ttgtatgtat ccagcggcgg cggcgcgcaa
    14701 cgaagctatg tccaagcgca aaatcaaaga agagatgctc caggtcatcg cgccggagat
    14761 ctatggcccc ccgaagaagg aagagcagga ttacaagccc cgaaagctaa agcgggtcaa
    14821 aaagaaaaag aaagatgatg atgatgaact tgacgacgag gtggaactgc tgcacgctac
    14881 cgcgcccagg cgacgggtac agtggaaagg tcgacgcgta aaacgtgttt tgcgacccgg
    14941 caccaccgta gtctttacgc ccggtgagcg ctccacccgc acctacaagc gcgtgtatga
    15001 tgaggtgtac ggcgacgagg acctgcttga gcaggccaac gagcgcctcg gggagtttgc
    15061 ctacggaaag cggcataagg acatgctggc gttgccgctg gacgagggca acccaacacc
    15121 tagcctaaag cccgtaacac tgcagcaggt gctgcccgcg cttgcaccgt ccgaagaaaa
    15181 gcgcggccta aagcgcgagt ctggtgactt ggcacccacc gtgcagctga tggtacccaa
    15241 gcgccagcga ctggaagatg tcttggaaaa aatgaccgtg gaacctgggc tggagcccga
    15301 ggtccgcgtg cggccaatca agcaggtggc gccgggactg ggcgtgcaga ccgtggacgt
    15361 tcagataccc actaccagta gcaccagtat tgccaccgcc acagagggca tggagacaca
    15421 aacgtccccg gttgcctcag cggtggcgga tgccgcggtg caggcggtcg ctgcggccgc
    15481 gtccaagacc tctacggagg tgcaaacgga cccgtggatg tttcgcgttt cagccccccg
    15541 gcgcccgcgc cgttcgagga agtacggcgc cgccagcgcg ctactgcccg aatatgccct
    15601 acatccttcc attgcgccta cccccggcta tcgtggctac acctaccgcc ccagaagacg
    15661 agcaactacc cgacgccgaa ccaccactgg aacccgccgc cgccgtcgcc gtcgccagcc
    15721 cgtgctggcc ccgatttccg tgcgcagggt ggctcgcgaa ggaggcagga ccctggtgct
    15781 gccaacagcg cgctaccacc ccagcatcgt ttaaaagccg gtctttgtgg ttcttgcaga
    15841 tatggccctc acctgccgcc tccgtttccc ggtgccggga ttccgaggaa gaatgcaccg
    15901 taggaggggc atggccggcc acggcctgac gggcggcatg cgtcgtgcgc accaccggcg
    15961 gcggcgcgcg tcgcaccgtc gcatgcgcgg cggtatcctg cccctcctta ttccactgat
    16021 cgccgcggcg attggcgccg tgcccggaat tgcatccgtg gccttgcagg cgcagagaca
    16081 ctgattaaaa acaagttgca tgtggaaaaa tcaaaataaa aagtctggac tctcacgctc
    16141 gcttggtcct gtaactattt tgtagaatgg aagacatcaa ctttgcgtct ctggccccgc
    16201 gacacggctc gcgcccgttc atgggaaact ggcaagatat cggcaccagc aatatgagcg
    16261 gtggcgcctt cagctggggc tcgctgtgga gcggcattaa aaatttcggt tccaccgtta
    16321 agaactatgg cagcaaggcc tggaacagca gcacaggcca gatgctgagg gataagttga
    16381 aagagcaaaa tttccaacaa aaggtggtag atggcctggc ctctggcatt agcggggtgg
    16441 tggacctggc caaccaggca gtgcaaaata agattaacag taagcttgat ccccgccctc
    16501 ccgtagagga gcctccaccg gccgtggaga cagtgtctcc agaggggcgt ggcgaaaagc
    16561 gtccgcgccc cgacagggaa gaaactctgg tgacgcaaat agacgagcct ccctcgtacg
    16621 aggaggcact aaagcaaggc ctgcccacca cccgtcccat cgcgcccatg gctaccggag
    16681 tgctgggcca gcacacaccc gtaacgctgg acctgcctcc ccccgccgac acccagcaga
    16741 aacctgtgct gccaggcccg accgccgttg ttgtaacccg tcctagccgc gcgtccctgc
    16801 gccgcgccgc cagcggtccg cgatcgttgc ggcccgtagc cagtggcaac tggcaaagca
    16861 cactgaacag catcgtgggt ctgggggtgc aatccctgaa gcgccgacga tgcttctgat
    16921 agctaacgtg tcgtatgtgt gtcatgtatg cgtccatgtc gccgccagag gagctgctga
    16981 gccgccgcgc gcccgctttc caagatggct accccttcga tgatgccgca gtggtcttac
    17041 atgcacatct cgggccagga cgcctcggag tacctgagcc ccgggctggt gcagtttgcc
    17101 cgcgccaccg agacgtactt cagcctgaat aacaagttta gaaaccccac ggtggcgcct
    17161 acgcacgacg tgaccacaga ccggtcccag cgtttgacgc tgcggttcat ccctgtggac
    17221 cgtgaggata ctgcgtactc gtacaaggcg cggttcaccc tagctgtggg tgataaccgt
    17281 gtgctggaca tggcttccac gtactttgac atccgcggcg tgctggacag gggccctact
    17341 tttaagccct actctggcac tgcctacaac gccctggctc ccaagggtgc cccaaatcct
    17401 tgcgaatggg atgaagctgc tactgctctt gaaataaacc tagaagaaga ggacgatgac
    17461 aacgaagacg aagtagacga gcaagctgag cagcaaaaaa ctcacgtatt tgggcaggcg
    17521 ccttattctg gtataaatat tacaaaggag ggtattcaaa taggtgtcga aggtcaaaca
    17581 cctaaatatg ccgataaaac atttcaacct gaacctcaaa taggagaatc tcagtggtac
    17641 gaaacagaaa ttaatcatgc agctgggaga gtcctaaaaa agactacccc aatgaaacca
    17701 tgttacggtt catatgcaaa acccacaaat gaaaatggag ggcaaggcat tcttgtaaag
    17761 caacaaaatg gaaagctaga aagtcaagtg gaaatgcaat ttttctcaac tactgaggca
    17821 gccgcaggca atggtgataa cttgactcct aaagtggtat tgtacagtga agatgtagat
    17881 atagaaaccc cagacactca tatttcttac atgcccacta ttaaggaagg taactcacga
    17941 gaactaatgg gccaacaatc tatgcccaac aggcctaatt acattgcttt tagggacaat
    18001 tttattggtc taatgtatta caacagcacg ggtaatatgg gtgttctggc gggccaagca
    18061 tcgcagttga atgctgttgt agatttgcaa gacagaaaca cagagctttc ataccagctt
    18121 ttgcttgatt ccattggtga tagaaccagg tacttttcta tgtggaatca ggctgttgac
    18181 agctatgatc cagatgttag aattattgaa aatcatggaa ctgaagatga acttccaaat
    18241 tactgctttc cactgggagg tgtgattaat acagagactc ttaccaaggt aaaacctaaa
    18301 acaggtcagg aaaatggatg ggaaaaagat gctacagaat tttcagataa aaatgaaata
    18361 agagttggaa ataattttgc catggaaatc aatctaaatg ccaacctgtg gagaaatttc
    18421 ctgtactcca acatagcgct gtatttgccc gacaagctaa agtacagtcc ttccaacgta
    18481 aaaatttctg ataacccaaa cacctacgac tacatgaaca agcgagtggt ggctcccggg
    18541 ctagtggact gctacattaa ccttggagca cgctggtccc ttgactatat ggacaacgtc
    18601 aacccattta accaccaccg caatgctggc ctgcgctacc gctcaatgtt gctgggcaat
    18661 ggtcgctatg tgcccttcca catccaggtg cctcagaagt tctttgccat taaaaacctc
    18721 cttctcctgc cgggctcata cacctacgag tggaacttca ggaaggatgt taacatggtt
    18781 ctgcagagct ccctaggaaa tgacctaagg gttgacggag ccagcattaa gtttgatagc
    18841 atttgccttt acgccacctt cttccccatg gcccacaaca ccgcctccac gcttgaggcc
    18901 atgcttagaa acgacaccaa cgaccagtcc tttaacgact atctctccgc cgccaacatg
    18961 ctctacccta tacccgccaa cgctaccaac gtgcccatat ccatcccctc ccgcaactgg
    19021 gcggctttcc gcggctgggc cttcacgcgc cttaagacta aggaaacccc atcactgggc
    19081 tcgggctacg acccttatta cacctactct ggctctatac cctacctaga tggaaccttt
    19141 tacctcaacc acacctttaa gaaggtggcc attacctttg actcttctgt cagctggcct
    19201 ggcaatgacc gcctgcttac ccccaacgag tttgaaatta agcgctcagt tgacggggag
    19261 ggttacaacg ttgcccagtg taacatgacc aaagactggt tcctggtaca aatgctagct
    19321 aactataaca ttggctacca gggcttctat atcccagaga gctacaagga ccgcatgtac
    19381 tccttcttta gaaacttcca gcccatgagc cgtcaggtgg tggatgatac taaatacaag
    19441 gactaccaac aggtgggcat cctacaccaa cacaacaact ctggatttgt tggctacctt
    19501 gcccccacca tgcgcgaagg acaggcctac cctgctaact tcccctatcc gcttataggc
    19561 aagaccgcag ttgacagcat tacccagaaa aagtttcttt gcgatcgcac cctttggcgc
    19621 atcccattct ccagtaactt tatgtccatg ggcgcactca cagacctggg ccaaaacctt
    19681 ctctacgcca actccgccca cgcgctagac atgacttttg aggtggatcc catggacgag
    19741 cccacccttc tttatgtttt gtttgaagtc tttgacgtgg tccgtgtgca ccagccgcac
    19801 cgcggcgtca tcgaaaccgt gtacctgcgc acgcccttct cggccggcaa cgccacaaca
    19861 taaagaagca agcaacatca acaacagctg ccgccatggg ctccagtgag caggaactga
    19921 aagccattgt caaagatctt ggttgtgggc catatttttt gggcacctat gacaagcgct
    19981 ttccaggctt tgtttctcca cacaagctcg cctgcgccat agtcaatacg gccggtcgcg
    20041 agactggggg cgtacactgg atggcctttg cctggaaccc gcactcaaaa acatgctacc
    20101 tctttgagcc ctttggcttt tctgaccagc gactcaagca ggtttaccag tttgagtacg
    20161 agtcactcct gcgccgtagc gccattgctt cttcccccga ccgctgtata acgctggaaa
    20221 agtccaccca aagcgtacag gggcccaact cggccgcctg tggactattc tgctgcatgt
    20281 ttctccacgc ctttgccaac tggccccaaa ctcccatgga tcacaacccc accatgaacc
    20341 ttattaccgg ggtacccaac tccatgctca acagtcccca ggtacagccc accctgcgtc
    20401 gcaaccagga acagctctac agcttcctgg agcgccactc gccctacttc cgcagccaca
    20461 gtgcgcagat taggagcgcc acttcttttt gtcacttgaa aaacatgtaa aaataatgta
    20521 ctagagacac tttcaataaa ggcaaatgct tttatttgta cactctcggg tgattattta
    20581 cccccaccct tgccgtctgc gccgtttaaa aatcaaaggg gttctgccgc gcatcgctat
    20641 gcgccactgg cagggacacg ttgcgatact ggtgtttagt gctccactta aactcaggca
    20701 caaccatccg cggcagctcg gtgaagtttt cactccacag gctgcgcacc atcaccaacg
    20761 cgtttagcag gtcgggcgcc gatatcttga agtcgcagtt ggggcctccg ccctgcgcgc
    20821 gcgagttgcg atacacaggg ttgcagcact ggaacactat cagcgccggg tggtgcacgc
    20881 tggccagcac gctcttgtcg gagatcagat ccgcgtccag gtcctccgcg ttgctcaggg
    20941 cgaacggagt caactttggt agctgccttc ccaaaaaggg cgcgtgccca ggctttgagt
    21001 tgcactcgca ccgtagtggc atcaaaaggt gaccgtgccc ggtctgggcg ttaggataca
    21061 gcgcctgcat aaaagccttg atctgcttaa aagccacctg agcctttgcg ccttcagaga
    21121 agaacatgcc gcaagacttg ccggaaaact gattggccgg acaggccgcg tcgtgcacgc
    21181 agcaccttgc gtcggtgttg gagatctgca ccacatttcg gccccaccgg ttcttcacga
    21241 tcttggcctt gctagactgc tccttcagcg cgcgctgccc gttttcgctc gtcacatcca
    21301 tttcaatcac gtgctcctta tttatcataa tgcttccgtg tagacactta agctcgcctt
    21361 cgatctcagc gcagcggtgc agccacaacg cgcagcccgt gggctcgtga tgcttgtagg
    21421 tcacctctgc aaacgactgc aggtacgcct gcaggaatcg ccccatcatc gtcacaaagg
    21481 tcttgttgct ggtgaaggtc agctgcaacc cgcggtgctc ctcgttcagc caggtcttgc
    21541 atacggccgc cagagcttcc acttggtcag gcagtagttt gaagttcgcc tttagatcgt
    21601 tatccacgtg gtacttgtcc atcagcgcgc gcgcagcctc catgcccttc tcccacgcag
    21661 acacgatcgg cacactcagc gggttcatca ccgtaatttc actttccgct tcgctgggct
    21721 cttcctcttc ctcttgcgtc cgcataccac gcgccactgg gtcgtcttca ttcagccgcc
    21781 gcactgtgcg cttacctcct ttgccatgct tgattagcac cggtgggttg ctgaaaccca
    21841 ccatttgtag cgccacatct tctctttctt cctcgctgtc cacgattacc tctggtgatg
    21901 gcgggcgctc gggcttggga gaagggcgct tctttttctt cttgggcgca atggccaaat
    21961 ccgccgccga ggtcgatggc cgcgggctgg gtgtgcgcgg caccagcgcg tcttgtgatg
    22021 agtcttcctc gtcctcggac tcgatacgcc gcctcatccg cttttttggg ggcgcccggg
    22081 gaggcggcgg cgacggggac ggggacgaca cgtcctccat ggttggggga cgtcgcgccg
    22141 caccgcgtcc gcgctcgggg gtggtttcgc gctgctcctc ttcccgactg gccatttcct
    22201 tctcctatag gcagaaaaag atcatggagt cagtcgagaa gaaggacagc ctaaccgccc
    22261 cctctgagtt cgccaccacc gcctccaccg atgccgccaa cgcgcctacc accttccccg
    22321 tcgaggcacc cccgcttgag gaggaggaag tgattatcga gcaggaccca ggttttgtaa
    22381 gcgaagacga cgaggaccgc tcagtaccaa cagaggataa aaagcaagac caggacaacg
    22441 cagaggcaaa cgaggaacaa gtcgggcggg gggacgaaag gcatggcgac tacctagatg
    22501 tgggagacga cgtgctgttg aagcatctgc agcgccagtg cgccattatc tgcgacgcgt
    22561 tgcaagagcg cagcgatgtg cccctcgcca tagcggatgt cagccttgcc tacgaacgcc
    22621 acctattctc accgcgcgta ccccccaaac gccaagaaaa cggcacatgc gagcccaacc
    22681 cgcgcctcaa cttctacccc gtatttgccg tgccagaggt gcttgccacc tatcacatct
    22741 ttttccaaaa ctgcaagata cccctatcct gccgtgccaa ccgcagccga gcggacaagc
    22801 agctggcctt gcggcagggc gctgtcatac ctgatatcgc ctcgctcaac gaagtgccaa
    22861 aaatctttga gggtcttgga cgcgacgaga agcgcgcggc aaacgctctg caacaggaaa
    22921 acagcgaaaa tgaaagtcac tctggagtgt tggtggaact cgagggtgac aacgcgcgcc
    22981 tagccgtact aaaacgcagc atcgaggtca cccactttgc ctacccggca cttaacctac
    23041 cccccaaggt catgagcaca gtcatgagtg agctgatcgt gcgccgtgcg cagcccctgg
    23101 agagggatgc aaatttgcaa gaacaaacag aggagggcct acccgcagtt ggcgacgagc
    23161 agctagcgcg ctggcttcaa acgcgcgagc ctgccgactt ggaggagcga cgcaaactaa
    23221 tgatggccgc agtgctcgtt accgtggagc ttgagtgcat gcagcggttc tttgctgacc
    23281 cggagatgca gcgcaagcta gaggaaacat tgcactacac ctttcgacag ggctacgtac
    23341 gccaggcctg caagatctcc aacgtggagc tctgcaacct ggtctcctac cttggaattt
    23401 tgcacgaaaa ccgccttggg caaaacgtgc ttcattccac gctcaagggc gaggcgcgcc
    23461 gcgactacgt ccgcgactgc gtttacttat ttctatgcta cacctggcag acggccatgg
    23521 gcgtttggca gcagtgcttg gaggagtgca acctcaagga gctgcagaaa ctgctaaagc
    23581 aaaacttgaa ggacctatgg acggccttca acgagcgctc cgtggccgcg cacctggcgg
    23641 acatcatttt ccccgaacgc ctgcttaaaa ccctgcaaca gggtctgcca gacttcacca
    23701 gtcaaagcat gttgcagaac tttaggaact ttatcctaga gcgctcagga atcttgcccg
    23761 ccacctgctg tgcacttcct agcgactttg tgcccattaa gtaccgcgaa tgccctccgc
    23821 cgctttgggg ccactgctac cttctgcagc tagccaacta ccttgcctac cactctgaca
    23881 taatggaaga cgtgagcggt gacggtctac tggagtgtca ctgtcgctgc aacctatgca
    23941 ccccgcaccg ctccctggtt tgcaattcgc agctgcttaa cgaaagtcaa attatcggta
    24001 cctttgagct gcagggtccc tcgcctgacg aaaagtccgc ggctccgggg ttgaaactca
    24061 ctccggggct gtggacgtcg gcttaccttc gcaaatttgt acctgaggac taccacgccc
    24121 acgagattag gttctacgaa gaccaatccc gcccgcctaa tgcggagctt accgcctgcg
    24181 tcattaccca gggccacatt cttggccaat tgcaagccat caacaaagcc cgccaagagt
    24241 ttctgctacg aaagggacgg ggggtttact tggaccccca gtccggcgag gagctcaacc
    24301 caatcccccc gccgccgcag ccctatcagc agcagccgcg ggcccttgct tcccaggatg
    24361 gcacccaaaa agaagctgca gctgccgccg ccacccacgg acgaggagga atactgggac
    24421 agtcaggcag aggaggtttt ggacgaggag gaggaggaca tgatggaaga ctgggagagc
    24481 ctagacgagg aagcttccga ggtcgaagag gtgtcagacg aaacaccgtc accctcggtc
    24541 gcattcccct cgccggcgcc ccagaaatcg gcaaccggtt ccagcatggc tacaacctcc
    24601 gctcctcagg cgccgccggc actgcccgtt cgccgaccca accgtagatg ggacaccact
    24661 ggaaccaggg ccggtaagtc caagcagccg ccgccgttag cccaagagca acaacagcgc
    24721 caaggctacc gctcatggcg cgggcacaag aacgccatag ttgcttgctt gcaagactgt
    24781 gggggcaaca tctccttcgc ccgccgcttt cttctctacc atcacggcgt ggccttcccc
    24841 cgtaacatcc tgcattacta ccgtcatctc tacagcccat actgcaccgg cggcagcggc
    24901 agcaacagca gcggccacac agaagcaaag gcgaccggat agcaagactc tgacaaagcc
    24961 caagaaatcc acagcggcgg cagcagcagg aggaggagcg ctgcgtctgg cgcccaacga
    25021 acccgtatcg acccgcgagc ttagaaacag gatttttccc actctgtatg ctatatttca
    25081 acagagcagg ggccaagaac aagagctgaa aataaaaaac aggtctctgc gatccctcac
    25141 ccgcagctgc ctgtatcaca aaagcgaaga tcagcttcgg cgcacgctgg aagacgcgga
    25201 ggctctcttc agtaaatact gcgcgctgac tcttaaggac tagtttcgcg ccctttctca
    25261 aatttaagcg cgaaaactac gtcatctcca gcggccacac ccggcgccag cacctgttgt
    25321 cagcgccatt atgagcaagg aaattcccac gccctacatg tggagttacc agccacaaat
    25381 gggacttgcg gctggagctg cccaagacta ctcaacccga ataaactaca tgagcgcggg
    25441 accccacatg atatcccggg tcaacggaat acgcgcccac cgaaaccgaa ttctcctgga
    25501 acaggcggct attaccacca cacctcgtaa taaccttaat ccccgtagtt ggcccgctgc
    25561 cctggtgtac caggaaagtc ccgctcccac cactgtggta cttcccagag acgcccaggc
    25621 cgaagttcag atgactaact caggggcgca gcttgcgggc ggctttcgtc acagggtgcg
    25681 gtcgcccggg cagggtataa ctcacctgac aatcagaggg cgaggtattc agctcaacga
    25741 cgagtcggtg agctcctcgc ttggtctccg tccggacggg acatttcaga tcggcggcgc
    25801 cggccgctct tcattcacgc ctcgtcaggc aatcctaact ctgcagacct cgtcctctga
    25861 gccgcgctct ggaggcattg gaactctgca atttattgag gagtttgtgc catcggtcta
    25921 ctttaacccc ttctcgggac ctcccggcca ctatccggat caatttattc ctaactttga
    25981 cgcggtaaag gactcggcgg acggctacga ctgaatgtta taagttcctg tccatccgca
    26041 cccactatct tcatgttgtt gcagatgaag cgcgcaagac cgtctgaaga taccttcaac
    26101 cccgtgtatc catatgacac ggaaaccggt cctccaactg tgccttttct tactcctccc
    26161 tttgtatccc ccaatgggtt tcaagagagt ccccctgggg tactctcttt gcgcctatcc
    26221 gaacctctag ttacctccaa tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg
    26281 gacgaggccg gcaaccttac ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa
    26341 accaagtcaa acataaacct ggaaatatct gcacccctca cagttacctc agaagcccta
    26401 actgtggctg ccgccgcacc tctaatggtc gcgggcaaca cactcaccat gcaatcacag
    26461 gccccgctaa ccgtgcacga ctccaaactt agcattgcca cccaaggacc cctcacagtg
    26521 tcagaaggaa agctagccct gcaaacatca ggccccctca ccaccaccga tagcagtacc
    26581 cttactatca ctgcctcacc ccctctaact actgccactg gtagcttggg cattgacttg
    26641 aaagagccca tttatacaca aaatggaaaa ctaggactaa agtacggggc tcctttgcat
    26701 gtaacagacg acctaaacac tttgaccgta gcaactggtc caggtgtgac tattaataat
    26761 acttccttgc aaactaaagt tactggagcc ttgggttttg attcacaagg caatatgcaa
    26821 cttaatgtag caggaggact aaggattgat tctcaaaaca gacgccttat acttgatgtt
    26881 agttatccgt ttgatgctca aaaccaacta aatctaagac taggacaggg ccctcttttt
    26941 ataaactcag cccacaactt ggatattaac tacaacaaag gcctttactt gtttacagct
    27001 tcaaacaatt ccaaaaagct tgaggttaac ctaagcactg ccaaggggtt gatgtttgac
    27061 gctacagcca tagccattaa tgcaggagat gggcttgaat ttggttcacc taatgcacca
    27121 aacacaaatc ccctcaaaac aaaaattggc catggcctag aatttgattc aaacaaggct
    27181 atggttccta aactaggaac tggccttagt tttgacagca caggtgccat tacagtagga
    27241 aacaaaaata atgataagct aactttgtgg accacaccag ctccatctcc taactgtaga
    27301 ctaaatgcag agaaagatgc taaactcact ttggtcttaa caaaatgtgg cagtcaaata
    27361 cttgctacag tttcagtttt ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt
    27421 caaagtgctc atcttattat aagatttgac gaaaatggag tgctactaaa caattccttc
    27481 ctggacccag aatattggaa ctttagaaat ggagatctta ctgaaggcac agcctataca
    27541 aacgctgttg gatttatgcc taacctatca gcttatccaa aatctcacgg taaaactgcc
    27601 aaaagtaaca ttgtcagtca agtttactta aacggagaca aaactaaacc tgtaacacta
    27661 accattacac taaacggtac acaggaaaca ggagacacaa ctccaagtgc atactctatg
    27721 tcattttcat gggactggtc tggccacaac tacattaatg aaatatttgc cacatcctct
    27781 tacacttttt catacattgc ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt
    27841 tatttttcaa ttgcagaaaa tttcaagtca tttttcattc agtagtatag ccccaccacc
    27901 acatagctta tacagatcac cgtaccttaa tcaaactcac agaaccctag tattcaacct
    27961 gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg ccttaaaaag
    28021 catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg tttcctgtcg
    28081 agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta agttcatgtc
    28141 gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa cgggcggcga
    28201 aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga tagggcggtg
    28261 gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc aggaatacaa
    28321 catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc gccttgtcct
    28381 ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc agcacagcac
    28441 cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca tggcggggac
    28501 cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc gacccctcat
    28561 aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca cctcccggta
    28621 ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc agctggccaa
    28681 aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac agtggagagc
    28741 ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg cacaacacag
    28801 gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa ccatatccca
    28861 gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac ctcgcacgta
    28921 actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat cctccagtat
    28981 ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg gagtgcgccg
    29041 agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat
    29101 atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc cggtctcgcc
    29161 gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca tccaggcgcc
    29221 ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata acatccacca
    29281 ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca cacacgggag
    29341 gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt atccaaaacc
    29401 tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc aaactctaca
    29461 gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa aaggcaaacg
    29521 gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc ctctataaac
    29581 attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct caatatatct
    29641 ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag agcgccctcc
    29701 accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca cagacctgta
    29761 taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc cttcgcaggg
    29821 ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc ccgccaggaa
    29881 ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg ctaaccagcg
    29941 tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa
    30001 aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa
    30061 ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca aacatgtctg
    30121 cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat tagaagcctg
    30181 tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca tgccggcgtg
    30241 accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct cggtcatgtc
    30301 cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg tcagtgctaa
    30361 aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca acattacagc
    30421 ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac ctgaaaaacc
    30481 ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttccacagc
    30541 ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc
    30601 gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag agcgagtata
    30661 tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac
    30721 gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc
    30781 cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa
    30841 gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca
    30901 aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat
    30961 gttacatcgt taattaacga tttcgaaccc ggggtaccga attcctcgag tctagaggag
    31021 catgcgacgt cgcaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
    31081 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    31141 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    31201 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
    31261 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    31321 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    31381 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    31441 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    31501 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    31561 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    31621 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    31681 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata caggtggcac
    31741 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    31801 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    31861 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    31921 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    31981 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    32041 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    32101 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    32161 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    32221 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    32281 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    32341 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    32401 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    32461 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    32521 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    32581 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    32641 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    32701 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    32761 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    32821 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    32881 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    32941 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    33001 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    33061 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    33121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    33181 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    33241 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    33301 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    33361 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    33421 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    33481 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    33541 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    33601 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    33661 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    33721 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    33781 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    33841 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
    33901 atttaggtga cactatagaa tactcaagct agttaattaa cgttaattaa catcatcaat
    33961 aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt ttgtgacgtg
    34021 gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt gatgttgcaa
    34081 gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg gtgtgcgccg
    34141 gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag taaatttggg
    34201 cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga agtgaaatct
    34261 gaataatttt gtgttactca tagcgcgtaa tctctagcat
//
