LOCUS       sequence-138107-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide 
            mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1),
            synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK573
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             673..690
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(743..761)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      757..804
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(874..891)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(874..890)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(883..905)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc actcaagcgg
       61 cgtggacctg ggcaccgaaa acctgtactt ccaatcgaac atgggcgcga acctgaactc
      121 cgcgtatgcg gccggcgtca aaattgccat cgtgatgggt agcaaatctg attgggcgac
      181 catgcagttt gcagctgacg tgctgaccac gctgaacgtg ccgttccatg ttgaagtggt
      241 tagcgcacac cgtacgccgg atcgcctgtt ttctttcgcc gaacaagcgg aagccaatgg
      301 cctgcatgtt attatcgcag gtgctggcgg tgcagcacac ctgccgggta tgctggcagc
      361 taaaaccctg gtcccggtgc tgggtgttcc ggtccagtca gcagcactgt cgggtgttga
      421 tagtctgtac tccattgtcc aaatgccgcg tggcatcccg gtgggtaccc tggcgattgg
      481 caaagcaggt gcagctaacg cagcactgct ggcagctcag atcctggcgc tgcatgacac
      541 ggaactggca ggtcgcctgg ctcactggcg tcaaagtcaa accgatgatg ttctggacaa
      601 cccggacccg cgtgaagaag cgtgacggat ccgaattcga gctccgtcga caagcttgcg
      661 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      721 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      781 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatg acccaatatc
      841 tagatgcatt cgcgaggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
      901 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      961 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
//
