LOCUS       sequence-138109-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, 
            Zheng N, Major MB, Moon RT
  TITLE     Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 
            protein through competitive binding to KEAP1 protein.
  JOURNAL   J Biol Chem. 2012 Feb 24;287(9):6539-50. Epub 2012 Jan 3.
  PUBMED    22215675
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2012-02-24"; volume: "287(9)"; pages: "6539-50. Epub 
            2012 Jan 3"
COMMENT     Sequence Label: NC16 pCDNA3.1 FLAG NRF2
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccgcc accatggact
      121 acaaggacga cgacgacaag ggcggcgcgc ctatgatgga cttggagctg ccgccgccgg
      181 gactcccgtc ccagcaggac atggatttga ttgacatact ttggaggcaa gatatagatc
      241 ttggagtaag tcgagaagta tttgacttca gtcagcgacg gaaagagtat gagctggaaa
      301 aacagaaaaa acttgaaaag gaaagacaag aacaactcca aaaggagcaa gagaaagcct
      361 ttttcgctca gttacaacta gatgaagaga caggtgaatt tctcccaatt cagccagccc
      421 agcacatcca gtcagaaacc agtggatctg ccaactactc ccaggttgcc cacattccca
      481 aatcagatgc tttgtacttt gatgactgca tgcagctttt ggcgcagaca ttcccgtttg
      541 tagatgacaa tgaggtttct tcggctacgt ttcagtcact tgttcctgat attcccggtc
      601 acatcgagag cccagtcttc attgctacta atcaggctca gtcacctgaa acttctgttg
      661 ctcaggtagc ccctgttgat ttagacggta tgcaacagga cattgagcaa gtttgggagg
      721 agctattatc cattcctgag ttacagtgtc ttaatattga aaatgacaag ctggttgaga
      781 ctaccatgg
//
