LOCUS       sequence-138112-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant 
            (AaPurE1-S57A,Y154F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK628
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             646..668
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(699..718)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(700..718)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattctcat ttatcttcgg taatgggaga gttggggaca
      121 gaggccgtct gcaatgcgcg ccatgtttca aggcgtgcgg caagggccgg gttaaacagt
      181 gcaagaatgg aggcggccag caaggcggca tttttggcgc cggatgcccc aatagccaaa
      241 gtgcccacag gcacgccgcc gggcatctgc acaatggaaa gcaggctgtc catgccttta
      301 agagcgcgag attctactgg aacacccagc acgggcaggc gtgtccatgc cgcacacata
      361 cccggcagat gtgcagctcc accggcccca gcaataatca cattcaggcc gcgttcggct
      421 gccgtacgcg cataatccgc caaccggtct ggcgtacgat gtgctgcaac aatgagggtt
      481 tcatgcggaa tttccagctc tgtcagcaat gcgtctgcat ggcgcatggt ttcccagtct
      541 gactggctgc ccatgataat gcccacaacg ggggcagagg ctgctttatc ttctaaagca
      601 ctggaagcag agggcagggg ggcggtttcg ctcatcatat gtatatctcc ttcttaaagt
      661 taaacaaaat tatttctaga gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat
      721 ttcgcgggat cgagatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
      781 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
      841 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
      901 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
      961 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtgga
//
