LOCUS       sequence-138122-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli glutamine synthetase with Y397F mutation 
            that prevents adenylation (EcGlnA-Y397F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK650
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgtccgct gaacacgtac tgacgatgct
       61 gaacgagcac gaagtgaagt ttgttgattt gcgcttcacc gatactaaag gtaaagaaca
      121 gcacgtcact atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt
      181 tgacggctcc tcgattggcg gctggaaagg cattaacgag tccgacatgg tgctgatgcc
      241 agacgcatcc accgcagtga ttgacccgtt cttcgccgac tccaccctga ttatccgttg
      301 cgacatcctt gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa
      361 gcgcgccgaa gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga
      421 acctgaattc ttcctgttcg atgacatccg tttcggatca tctatctccg gttcccacgt
      481 tgctatcgac gatatcgaag gtgcatggaa ctcctccacc caatacgaag gtggtaacaa
      541 aggtcaccgt ccggcagtga aaggcggtta cttcccggtt ccaccggtag actcggctca
      601 ggatattcgt tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca
      661 tcaccacgaa gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac
      721 caaaaaagct gacgaaattc agatctacaa atatgttgtg cacaacgtag cgcaccgctt
      781 cggtaaaacc gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca
      841 ctgccacatg tctctgtcta aaaacggcgt taacctgttc gcaggcgaca aatacgcagg
      901 tctgtctgag caggcgctgt actacattgg cggcgtaatc aaacacgcta aagcgattaa
      961 cgccctggca aacccgacca ccaactctta taagcgtctg gtcccgggct a
//
