LOCUS       sequence-138123-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli glutamine synthetase with Y397F mutation 
            that prevents adenylation (EcGlnA-Y397F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK650
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agttagacgc
       61 tgtagtacag ctcaaactct accggatgcg gagtcatacg cacgcggtca tcttcttcgc
      121 gacgcagagc gatgtacgca tcaattgctt cgtcagtgaa cacgccaccg gctttcagga
      181 actcgcggtc cagatccagt tcgttcagtg cttcttccag agagcctgca acctgtggga
      241 tctctttcgc ttcttctggc ggcaggtcaa acaggttttt gtccatggct tcgcccggat
      301 ggatcttgtt cttgatacca tcaagaccgg ccatcagcag ggcagcaaag cacaggtacg
      361 ggttagctgc cggatccggg aaacgtactt cgatacgacg tgctttcgga gaagaaacca
      421 ccggaatacg gatagacgca gaacggttac gcgcagagta agccagcatt accggtgctt
      481 catagcccgg gaccagacgc ttataagagt tggtggtcgg gtttgccagg gcgttaatcg
      541 ctttagcgtg tttgattacg ccgccaatgt agtacagcgc ctgctcagac agacctgcgt
      601 atttgtcgcc tgcgaacagg ttaacgccgt ttttagacag agacatgtgg cagtgcatac
      661 cggagccgtt atcaccgaac atcggttttg gcataaaggt cgcggtttta ccgaagcggt
      721 gcgctacgtt gtgcacaaca tatttgtaga tctgaatttc gtcagctttt ttggtcatgg
      781 tattgaagcg ggtagccact tcgttctgac cagcagtcgc tacttcgtgg tgatgggctt
      841 caaccaccag acccatctgt tccatcacca gacacatttc agaacgaata tcctgagccg
      901 agtctaccgg tggaaccggg aagtaaccgc ctttcactgc cggacggtga cctttgttac
      961 caccttcgta ttgggtggag gagttccatg
//
