LOCUS       sequence-138126-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains sgRNA which targets GFPmut3b. Under an aTc inducible 
            promoter. Suicide vector inserts into slr0230 site of Synechocystis.
            Carries kanamycin resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0230-PL31-sgRNANT1-KmR
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    97..118
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctatttctag ctctaaaaca attcttgttg aattagatgg taccacacat tatatctatc
       61 actgataggg atgtcaatct ctatcactga tagggactct agaagcggcc gcgaattcca
      121 tcagccacag ggtaaatggt tgtgcccaac ttttgagcca catcggccac ccggaggtca
      181 tccaagaagc gagtatcatc atgtttcaac atcagagcag gcaatagaat accatcccct
      241 aaatctctgc ctgccaattt agcgattaaa tcctgccccg tcaatagtcc cgtcaccgta
      301 atttcctgac cccaatagtc actgtttaag ggcgctaaat taactgttaa accctttacc
      361 tgatttaact gttccaccag tagctcaaac gcttgttcta cagcattgcc cactacccag
      421 gtcaacgtct tggcttcggc gatcgccggg gggagaaatt ccgcagcttg ttgttgaaac
      481 tccttaatga attgacggat agaccctacc ccgttgccaa tttggggata gtcttcgtaa
      541 tggctttccg gtggcaaagg ttgacgggca atgagaaacc attcatcggc caaccaaaca
      601 aagttaccac ctagttgctg ggcaaattcc ttttgcaaag cttgaacctg ggcaattacc
      661 tccgtggctt tagcttgacc aacggggctt aactcatctt cctggggacg aaaacgggtt
      721 agacccaccg gcaccaccgc cactgaaatc accgctgggg tttccccttg gtgaaactgg
      781 gctaagtccc tcaaagtttg ctctaagtgc ttaccatcat taacaccagg acaaaccacc
      841 acctgggcgt ggatctgtaa tcttctagct tggaaccaag ccaactggtc aagaatctga
      901 ccggcccgat gattttttaa caatctttcc cgcaccgatg cttccgtggc atggatggaa
      961 acgta
//
