LOCUS       sequence-138127-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT),
            synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK694
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(5..194)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     206..224
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     242..258
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     243..259
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             584..592
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
       61 acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta
      121 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt
      181 cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg tgacataact aattacatga
      241 ctcgaggtcg acggtatcga taagctttta ccattccaat tcaactgggt acttaccagt
      301 caaacaagca gtacaatgac cggacttttc gaaacattcc aaaccgttac cattttcttg
      361 gatcatgatg tcgtgcttct tttccttttg cttcttgaac ttgatacctt cttgaacaga
      421 agaaaccaaa ccttcaacag ataagtaaac aacggagtta gcacctaagt attcagctaa
      481 gtgatcgaac tctggtttgt tggcgattaa ttcctcctta gttggaatgt tgatacccat
      541 gaaacatggg tacttgattg gtggagaagc aactctaata tggacctcct tagcaccaga
      601 ttccttcaac aacttaataa ttggagagat ggtgttacca cgaacaatgg aatcgtcgac
      661 taaaacaatt ctcttaccct tgaagttgtc ggacaaaaca ccgaactttt tagcgatacc
      721 caattgacgc aatctcatat ttggttgaat gaaagttcta ccaacatatc tgttcttaca
      781 caaaacttcg acgtatggca aaccacactt accagcgtaa gctaaagcgg ctggggtagc
      841 ggattcaggg acagtagaaa ccaagtcagc gtcgactgga gcttcgatag ccaattgttg
      901 accacatctg tatctgacgg tataaaccat ttgatcttcg aacatggagt caggtctggc
      961 aaagtaaacg tattcgaaga tgcagaaagc gactgggtta ccttcagatc tggagatgat
     1021 gtccaaagtt tgaacgttgt gtctagaaa
//
