LOCUS       sequence-138133-         114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains sgRNA which targets GFPmut3b. Under an aTc inducible 
            promoter. Suicide vector inserts into slr0230 site of Synechocystis.
            Carries kanamycin resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 114)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0230-PL22-sgRNANT1-KmR
FEATURES             Location/Qualifiers
     source          1..114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..67
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    74..92
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    93..114
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ttctagctct aaaacaattc ttgttgaatt agatggtagc tcccagtatc tctatcactg
       61 atagggatgt caatctctat cactgatagg gactctagaa gcggccgcga attc
//
