LOCUS       sequence-138136-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human LC3A/MAP1LC3A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pGex-4T-2_LC3A
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     602..624
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             651..668
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             669..1031
                     /codon_start=1
                     /gene="MAP1LC3A"
                     /product="human LC3 protein"
                     /label=LC3A
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKD
                     EDGFLYMVYASQETFGF"
     RBS             719..727
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc
       61 ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca
      121 aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta
      181 aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg
      241 gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat
      301 acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta
      361 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
      421 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
      481 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
      541 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
      601 agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat ctggttccgc
      661 gtggatccat gccctcagac cggcctttca agcagcggcg gagcttcgcc gaccgctgta
      721 aggaggtaca gcagatccgc gaccagcacc ccagcaaaat cccggtgatc atcgagcgct
      781 acaagggtga gaagcagctg cccgtcctgg acaagaccaa gtttttggtc ccggaccatg
      841 tcaacatgag cgagttggtc aagatcatcc ggcgccgcct gcagctgaac cccacgcagg
      901 ccttcttcct gctggtgaac cagcacagca tggtgagtgt gtccacgccc atcgcggaca
      961 tctacgagca ggagaaagac gaggacggct tcctctatat ggtctacgcc tcccaggaaa
     1021 ccttcggctt ctaagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt
     1081 gatgacgg
//
