LOCUS       sequence-138138-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 del7-GloA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 ORFs associated with functional 
            complementation of an alanine racemase (alr-, dadX-) auxotroph
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK268
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             574..596
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    611..635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(635..654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(636..654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttgtcgac ggagctcgaa ttcagcttga tgcatattcc gacccagctg tgcagaaagc
      121 cgccaaaacc atcaatgatt cactaaaccg ccttacagcg cggctgaagc agcgcccgca
      181 aggctgaaca gcgctttatt cagcgcgttc aatcagctca accttgtagc catcggggtc
      241 ttctacaaag gcaatcacgg tggtgccaaa ttttaccggc ccagcttccc gcgttacctt
      301 gccaccaccg gcccgcacac gttcaaccat ttccgccaca ttgggcacac ccacggcaaa
      361 atggccaaag cctgtgccca gctcgtagcc atcatccttg ccccagttat aggtcagctc
      421 aatttctgcc tgtccggccg cgttatctgc atagcctata aacaccagcg tataacgccc
      481 ttccggcaca tccttccggc ggagttcgtg catgcccagc agtttgtaaa acgccagact
      541 tttttccaga ttacgcaccc gcaccatggt atatctcctt cttaaagtta aacaaaatta
      601 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      661 cgggatcgag atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
      721 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
      781 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
      841 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
      901 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
      961 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     1021 ccataccgcc agttgtttac cctc
//
