LOCUS       sequence-138139-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK402
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             550..567
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(620..638)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      634..681
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(805..824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgac cacccactcc atagatttca
      121 gaatctatta tgaagatacg gatgctggcg gcattgtgta ccatgcacgg tatctggctt
      181 ttgcggaacg cgcgcgtacc gaggcgattc gtagcctagg tacgccggca tcacgtttgc
      241 aggaagaata tgggctggtt tttgtggtgc atgacgccca tcttgcttac aaaatgcccc
      301 tgaagctgga tgacattgta acagttacca cccgcttgat tgacctgaaa gccgcaagct
      361 gccgactgga tcagctttgc acgcgcggtg atgatgtatg cgcacaggtt gaagttggtc
      421 tggcctgtgt gaaggtatca gatgggcgtc ctgcccgatt tccgccgtta tggcgtgatc
      481 tgttgcaaaa attggcacca aagggatgag aattcgagct ccgtcgacaa gcttgcggcc
      541 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      601 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      661 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      901 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      961 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1021 tcttgtt
//
