LOCUS       sequence-138140-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK402
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(505..522)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(532..549)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             568..590
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    605..629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(629..648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(630..648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(732..751)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattctca tccctttggt gccaattttt gcaacagatc
      121 acgccataac ggcggaaatc gggcaggacg cccatctgat accttcacac aggccagacc
      181 aacttcaacc tgtgcgcata catcatcacc gcgcgtgcaa agctgatcca gtcggcagct
      241 tgcggctttc aggtcaatca agcgggtggt aactgttaca atgtcatcca gcttcagggg
      301 cattttgtaa gcaagatggg cgtcatgcac cacaaaaacc agcccatatt cttcctgcaa
      361 acgtgatgcc ggcgtaccta ggctacgaat cgcctcggta cgcgcgcgtt ccgcaaaagc
      421 cagataccgt gcatggtaca caatgccgcc agcatccgta tcttcataat agattctgaa
      481 atctatggag tgggtggtca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg
      541 atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa attatttcta
      601 gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat
      661 cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
      721 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
      781 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
      841 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
      901 actactgggc tgcttcctaa tgcaggagtc gcataaggga
//
