LOCUS       sequence-138141-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E. coli AlkB absent the first 11 amino acids and with a 
            C-terminal histidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Frick LE, Delaney JC, Wong C, Drennan CL, Essigmann JM
  TITLE     Alleviation of 1,N6-ethanoadenine genotoxicity by the Escherichia 
            coli adaptive response protein AlkB.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):755-60. Epub 2007 Jan 
            9.
  PUBMED    17213319
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-01-16"; volume: "104(3)"; pages: 
            "755-60. Epub 2007 Jan 9"
COMMENT     Sequence Label: pET24a-AlkB deltaN11
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     272..291
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     375..394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        375..393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    394..418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             433..455
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
       61 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
      121 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
      181 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
      241 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
      301 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
      361 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
      421 tctagaaata attttgttta actttaagaa ggagatatac atatgcaaga gccactggcg
      481 gctggtgcgg taattttacg gcgttttgct tttaacgctg cggagcaact gatccgcgat
      541 attaatgacg ttgccagcca gtcgccgttt cgccagatgg tcacccccgg gggatatacc
      601 atgtcggtgg cgatgaccaa ctgtgggcat ctgggctgga cgacccatcg gcaaggttat
      661 ctctattcgc ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc acagagtttt
      721 cataatttat gtcaacgtgc ggctacggcg gcgggctatc cagatttcca gccagatgct
      781 tgtcttatca accgctacgc tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa
      841 ccggatctgc gcgcgccaat tgtttctgtt tctctgggct tacccgcga
//
