LOCUS       sequence-138142-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E. coli AlkB absent the first 11 amino acids and with a 
            C-terminal histidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Frick LE, Delaney JC, Wong C, Drennan CL, Essigmann JM
  TITLE     Alleviation of 1,N6-ethanoadenine genotoxicity by the Escherichia 
            coli adaptive response protein AlkB.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):755-60. Epub 2007 Jan 
            9.
  PUBMED    17213319
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-01-16"; volume: "104(3)"; pages: 
            "755-60. Epub 2007 Jan 9"
COMMENT     Sequence Label: pET24a-AlkB deltaN11
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..49
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(102..119)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagcc
       61 aactcagctt cctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc
      121 tcgaggcctt gaaaatatag gttttctttt ttacctgcct gacggaatgt caggttgtag
      181 cggcagtcga tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac
      241 agccgcgatt caccgcccca taccaccaca tcgccatgtt ccaacaacaa acgtttgagc
      301 ggatcatttc gtttcaggcc gccaaattga aaaatcgcgg gtaagcccag agaaacagaa
      361 acaattggcg cgcgcagatc cggttcgtct ttatcctgat gcagcgacag tttcgcgcca
      421 ggagcgtagc ggttgataag acaagcatct ggctggaaat ctggatagcc cgccgccgta
      481 gccgcacgtt gacataaatt atgaaaactc tgtggcatgg cgggccacgg tttatttgtt
      541 tgcggatcaa tgggcgaata gagataacct tgccgatggg tcgtccagcc cagatgccca
      601 cagttggtca tcgccaccga catggtatat cccccggggg tgaccatctg gcgaaacggc
      661 gactggctgg caacgtcatt aatatcgcgg atcagttgct ccgcagcgtt aaaagcaaaa
      721 cgccgtaaaa ttaccgcacc agccgc
//
