LOCUS       sequence-138143-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 del7-GloA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 ORFs associated with functional 
            complementation of an alanine racemase (alr-, dadX-) auxotroph
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK268
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             555..572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(625..643)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      639..686
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(810..829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggtgcggg tgcgtaatct ggaaaaaagt
       61 ctggcgtttt acaaactgct gggcatgcac gaactccgcc ggaaggatgt gccggaaggg
      121 cgttatacgc tggtgtttat aggctatgca gataacgcgg ccggacaggc agaaattgag
      181 ctgacctata actggggcaa ggatgatggc tacgagctgg gcacaggctt tggccatttt
      241 gccgtgggtg tgcccaatgt ggcggaaatg gttgaacgtg tgcgggccgg tggtggcaag
      301 gtaacgcggg aagctgggcc ggtaaaattt ggcaccaccg tgattgcctt tgtagaagac
      361 cccgatggct acaaggttga gctgattgaa cgcgctgaat aaagcgctgt tcagccttgc
      421 gggcgctgct tcagccgcgc tgtaaggcgg tttagtgaat cattgatggt tttggcggct
      481 ttctgcacag ctgggtcgga atatgcatca agctgaattc gagctccgtc gacaagcttg
      541 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      601 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      661 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      721 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaata
//
