LOCUS       sequence-138145-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            N-terminal His tag and Ala368 deletion (H6AaAlr-A368del).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK344
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttca agccgttgca taaacgcgat gataacgccg ccccagagat gtcagtactt
      121 cgtagcctat tgtgccttct gctttggcca cagcatctac gccgtaagac tcattcaaaa
      181 gttccaccat gctatcttct tccagcagcc cttcgggcac tgcggaaaga tcaactgtca
      241 tggaatccat agagattcgg cccagaattg gtagccgata tcctttatac caagcgcatc
      301 ctgcagcagc gcccgcacgg gcaaatccat cagcatatcc aaccgcaatg gtcgcaacac
      361 ggcatgggcc agtcgcttgc cacgtaagcc catatcccac ccgatcccct ttggataaag
      421 tgcgaatttg cagaatatgc gtttgcaacc ccacaacagg cttaagtgga ttaacaccat
      481 cattctgcgg cgctacgcca tacaaagcgg caccagggcg cacgagatca aaatgaaatg
      541 gttgccccag aaaaattcca gaagatgcag aaagacttaa agctgccttt ggcaacatgc
      601 gtgcatagtt ctcaaaccgt tcacgctgca tggcattggc tgcgttttct ggcgtatctg
      661 cacaggccag atgactcata accagactga cagataaccc ctccaaaagt tcaggagtgg
      721 ccatcagttt atggatatca gcctctgaaa ggccaaaacg agacataccc gtatccagtt
      781 gaatggccac atccagcggc ttatccattt tggctgccag atcccgcaaa acagccacct
      841 gttttaggct atttaaaact ggccgcaaat tattgcgtac gcaaacctca gcagcatccg
      901 gccgtggacc atgcaacaca gttactgatg tgtggggtga aagatatggc ctaagggcta
      961 gcccttcatc cacatgtgcg acaaaaaaat gttt
//
