LOCUS       sequence-138152-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK403
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             214..225
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgccaaaat cagtagttaa tcccgaattt
      121 cttcccactc ttgagaaaat gccatctttt gatggtcttt catcgctctc acttccagaa
      181 gtgcgagagg tttttctaac atctgtgcgc cttattgaag ggcggcctct gccagatgtt
      241 gtaacgcggg aagaatacgt gccggggttg aataatgccc cagatgtacg cgtgattgtt
      301 tatacgccac gtaatgtaca gcccaatgca ccggccatgg tttatattca tggtggtggc
      361 cttgtttctg gtcatcctga agtggatgat cccaaatgcc agaaactggc cagtgatctg
      421 ggctttcagg ttttctccgt agattaccgc ctggcaccag aagtgaaata tcccggtgca
      481 attgaagatt gttatgccgt gctgaaatgg gtgcatgaca atgctagcaa gcttggcatc
      541 tcgcgtgata aggttgcggt tgcgggtgaa agtgcaggcg gtggtttaag tgcagcgcta
      601 agcctgatgg cgcgtgacaa aaaagaatac aagctggctt ttcagttgct catttacccc
      661 atgctggatg accgcactgc aataaatagt gatccgggcg ccaattttgg tgaatttgtc
      721 tggacacggt cggccaacaa atatgcatgg caggcgtatc tgggggataa agcaggtggg
      781 ccgcaaacac caccttatgc cgctgcgggc cgcgcaactg atctggcagg tctgccccct
      841 acttttattg gtgtgggatc aatggatctt ttcttgaagg aagatctgga ttacgcaact
      901 cgtctgatgg ctgcggctgt gccaacagaa gttatggtag tgcccggcgc gtatcatgtt
      961 tttgacatgt atgtgccaga tgctgaactt tctcgcaggt ttatggatgc ctactgc
//
