LOCUS       sequence-138164-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK404
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             614..631
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(684..702)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      698..745
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(869..888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgtcagaaa cctatggcac cggccagaag
      121 gaacagcact gcatgccccg ctcagaaaat tcccaagccc caaccgatat ccccaccatc
      181 cgcgtggtgg ccatgcccac agacaccaac ccggcgggag acgtgtttgg cgggtggatt
      241 gtttctcaga tggatcttgc agccggcacc acggcggcgt ttcgggccaa tggccggtgc
      301 gccaccattg ccattaacag ccttgtgttt atggaacccg tgatggtggg ggatgaagtg
      361 agcatttaca cccgcatttt gcgcgcgggg cgtacatcgc tcaccattca tgttgaaaca
      421 tggcgccggg cccgccacac gcatctgaca tccaaggtga cggaaggcga gtttattttt
      481 gtggcgctgg atgaaaaccg ccgccctcgc cagctgccac ccccgcagcc gggtgatgaa
      541 ctgccagaaa ttgtaccccc caacgcacgc taggaattcg agctccgtcg acaagcttgc
      601 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      661 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      721 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      781 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1021 cc
//
