LOCUS       sequence-138168-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK405
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgccaaaga atgtcgtaaa tccggaattt
      121 ctgcccgttc tggcaaaaat gccgtctttc gacggtcttt cggatcgttc actgccggaa
      181 gtacgggaaa tcttccttac ctctatccgg ctcatggaag gcagaccttc gccagatgtc
      241 gatacacggg aagaatacgt ccccggcctc aacaaggcgc ccgacgtgcg tgtcctcgtc
      301 tacagccccc gaaaagtcgc ccccaacgct cctgccatgg tctatattca cggcggcggt
      361 ctcatctccg gccacccgga agtggacgat ccgaaatgcc agatcctagc caaccgtctc
      421 ggcatgcaca tcttctcggt cgattatcgt ctcgctccgg aagtgaaata tcccggcgcc
      481 atcgaagact gttacgcagt tctcaaatgg gtgaacgaaa acgcagcccg cttgggtatc
      541 aaccgcgata aagtcgttgt cgccggagaa agcgcgggcg gcggcctcag cgcggctctc
      601 gccctcatgg cccgtgatcg cggcgaatac aaactcgctt gtcagatcct catctacccg
      661 atgcttgatg accgcacggc gataaaacac gaacctgccc ccgcgttcgg tgaatatgtc
      721 tggacccgct cagccaacaa atacgcctgg cagtgctacc tcggagacgc agctggcggc
      781 gataaaaccc cggactatgc agccgccgga cgcgcaacaa acctcgctgg tctgccgccc
      841 accttcatcg gggtcggctc catggaccta tttctatgcg aagacctcga ttacgccacc
      901 cgcctgatgg ctgccggcgt tcccgtcgaa gtcttcgtgg tgcccggcgc ctaccacgtc
      961 ttcgatatgt tcgtccctga cgccgccctc tcaaaacgct tcatggacgc
//
