LOCUS       sequence-138170-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 citrate synthase with N-terminal 
            His6 tag (H6AaAarA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK217
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgagcgcgt cgcagaaaga aggtaagcta
      121 tctaccgcta ccatttcggt tgatggaaaa tccgccgaaa tgcctgtgct ttcaggcact
      181 ctgggaccgg atgttatcga catccgcaaa cttccggcgc aactgggcgt tttcacgttc
      241 gacccaggtt acggggaaac agcggcctgc aacagcaaaa tcacctttat tgatggtgat
      301 aaaggcgttc tgctgcaccg tggttaccct attgcgcagc tggccgaaaa tgcttcctac
      361 gaagaagtta tttatctgct tttgaatggc gaactgccca acaaggcgca gtacgacacc
      421 ttcaccaaca cccttacaaa ccatacgctg ctgcacgagc agatccgtaa cttctttaac
      481 ggcttccggc gtgatgccca cccaatggcc attctgtgtg gtacggttgg ggctttgtct
      541 gccttctacc cagatgccaa cgatattgcc attcccgcca atcgggatct ggccgccatg
      601 cggctgattg ccaaaatccc aaccattgcg gcatgggctt acaaatacac gcagggtgaa
      661 gcctttatct acccgcggaa tgatctgaac tacgcagaaa acttcctgtc catgatgttc
      721 gcgcgcatgt ccgaacctta caaggtcaac cctgttctgg cccgcgccat gaaccggatt
      781 ctgattctgc atgccgatca tgagcagaat gcctctacct ccaccgtacg tctggctggt
      841 tctacagggg ccaatccgtt tgcctgtatt gctgcgggca ttgccgctct gtggggacct
      901 gcacatggtg gcgcaaacga agctgtgctg aaaatgctgg cccgtattgg caagaaagaa
      961 aatattcctg cctttatcgc acag
//
