LOCUS       sequence-138174-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Thermoplasma acidophilum citrate synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Kappock
  TITLE     Additional Thermoplasma acidophilum citrate synthase (TpCS) 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK436
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtcact ttctttcagc
       61 gatgggtacg tactttctct ctgcaggacc gacgtaaacg gctctcggcc taatcagtct
      121 ctgctgttcc tccacgtact ctatgaagtg tgcctgccac cctgtaaccc tggatagcgc
      181 gaagagcgcc gtgtatatgt tgttcctcag cgggaatcct atggacatgt atactattcc
      241 ggagaagtaa tccgtgttcg gatatatgcc ctttgaaccg aatgccttga tgccaaagtc
      301 ttcaagcttc gtggcgatct catatacctt gtgcacctct ggcttcttgc tggagagttt
      361 ctcagcgatg cccttgaata tctttgccct tggatcgtag gtcttgtata ccctgtggcc
      421 gaagcccatg agtctcttct ttccgtttat tatgttgtcg ttgaaccact tctccaccat
      481 ggcgggatcc tttatctcat cgaactgcgc tattgctgcc tcagctgcac cgccatgcag
      541 cggacccttg agggccgcaa gagctgcagt tatgccggag tacatgtctg agagggttga
      601 taccgcgacc aagcctgccg tggtagatgc aggcacctca tggtctgtgt agagaatcag
      661 tgccgtattc atggcgtcta tctcttcctt tgttgccttc cttccaaacg cagcgttcag
      721 gaagctctca gcgtagctgt ctgatggttt cggaagctcg gccggcatgt tcattatgtg
      781 cctgtacacg ttcacggtta tcgctgacat cctgccgatc atttctgctg caacgtctct
      841 gtccgtatcc ttgttccact tgaactttgt ctccgatgct gccatggctg caaccgcggc
      901 catctgcatt gccacagcat ccgattccct tggcagctgc cttatggcgt ttatgacaaa
      961 atctgg
//
