LOCUS       sequence-138175-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK284 (Stag-3FLAG-Hygro)
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg
       61 aagcttaccg ctagcatgga tagatccgga aagcctgaac tcaccgcgac gtctgtcgag
      121 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
      181 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
      241 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
      301 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
      361 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
      421 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
      481 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
      541 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
      601 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
      661 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
      721 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
      781 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
      841 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
      901 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
      961 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     1021 agcgcggccg tctggaccga
//
