LOCUS       sequence-138177-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Thermoplasma acidophilum citrate synthase pseudo-dimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Kappock
  TITLE     Additional Thermoplasma acidophilum citrate synthase (TpCS) 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK439
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtcacttt
       61 ctttcagcga tgggtacgta ctttctctct gcaggaccga cgtaaacggc tctcggccta
      121 atcagtctct gctgttcctc cacgtactct atgaagtgtg cctgccaccc tgtaaccctg
      181 gatagcgcga agagcgccgt gtatatgttg ttcctcagcg ggaatcctat ggacatgtat
      241 actattccgg agaagtaatc cgtgttcgga tatatgccct ttgaaccgaa tgccttgatg
      301 ccaaagtctt caagcttcgt ggcgatctca tataccttgt gcacctctgg cttcttgctg
      361 gagagtttct cagcgatgcc cttgaatatc tttgcccttg gatcgtaggt cttgtatacc
      421 ctgtggccga agcccatgag tctcttcttt ccgtttatta tgttgtcgtt gaaccacttc
      481 tccaccatgg cgggatcctt tatctcatcg aactgcgcta ttgctgcctc agctgcaccg
      541 ccatgcagcg gacccttgag ggccgcaaga gctgcagtta tgccggagta catgtctgag
      601 agggttgata ccgcgaccaa gcctgccgtg gtagatgcag gcacctcatg gtctgtgtag
      661 agaatcagtg ccgtattcat ggcgtctatc tcttcctttg ttgccttcct tccaaacgca
      721 gcgttcagga agctctcagc gtagctgtct gatggtttcg gaagctcggc cggcatgttc
      781 attatgtgcc tgtacacgtt cacggttatc gctgacatcc tgccgatcat ttctgctgca
      841 acgtctctgt ccgtatcctt gttccacttg aactttgtct ccgatgctgc catggctgca
      901 accgcggcca tctgcattgc cacagcatcc gattcccttg gcagctgcct tatggcgttt
      961 atgacaaaat ctggtatctt ataacccttc tggacggttt cct
//
