LOCUS       sequence-138183-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mClover using CRIPSR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK278 (mClover-Hygro)
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
       61 gaagcttacc gctagcatgg atagatccgg aaagcctgaa ctcaccgcga cgtctgtcga
      121 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
      181 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
      241 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
      301 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
      361 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
      421 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
      481 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
      541 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
      601 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
      661 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
      721 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
      781 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
      841 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
      901 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
      961 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     1021 aagcgcggcc gtctggaccg atggctgtgt agaagtact
//
