LOCUS       sequence-138184-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Janthinobacterium lividum violacein biosynthesis protein 
            VioA with a N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Kappock
  TITLE     Janthinobacterium lividum violacein biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK261
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgagcacgt attctgacat ttgcatcgtt
      121 ggcgccggca taggaggctt gacttgcgcc aacaacctga tcgacgccgc cgccggcagg
      181 aacctgcgca tccgcgtatt cgacctgaat gccaccgtag gcggccgcat ccagtcgcgg
      241 aaaatagatg gcgaggaaat cgccgaactc ggcgccgccc gctactcgcc gcagctgcat
      301 ccgcatttcc agcaactcat gcagggcagc ggcttgccgc atgcggtcta cccgttcacc
      361 gaggtcatct cccacgatag cgtgctggaa gagctgaagg caacgctgga tgagctgagc
      421 ccgatgctga aaatgcatcc gaacgactcc ttcctcgagt tcgtcagcca ttacctgggc
      481 gccgccaagg ccagccacat catcaaggcg accggctatg acgccctgct gctgccgatg
      541 gtgtcggcgg ccatggccta cgacatcatc aagaagcacc cggaaacgca gcactttacg
      601 gaaaacgccg ccaaccagtg gcactacgcc accgacggct accacgaatt gctgtgccag
      661 ttgcagcacc aggcccaggt cgccggggtg gaattcaggc tcgaacaccg cttgctgtcc
      721 gttgaaaaat cgggcgccga ccatgtgctc gccttcagcc accatggcga cacgcagatg
      781 caccgcacgc gccatctggt gatggccatc ccgccgtccg ccatgccgcg cctgaacctg
      841 gatttcccga acgcctggag tccgttccaa tacgactcgc tgcccctgtt caagggattc
      901 ttcacgttcg acacagcctg gtgggatgcg ctggggctga ccgacaaggt gctgatggcg
      961 gcaaatcccc tgcgcaagat ctacttcaag a
//
