LOCUS       sequence-138185-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Janthinobacterium lividum violacein biosynthesis protein 
            VioA with a N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Kappock
  TITLE     Janthinobacterium lividum violacein biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK261
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      691..700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 catgcgcgct cggtcgagga agtgatgaag gtatcgttgg cagcttcttc ttccgtccgt
      121 tgatcgaggc gctgcagcaa caggccgctg gcgtgctggg cgctgatcag gctgccttcc
      181 atccagccgc aatgcgcggt ataggcatcc gagcaggaga tgatgccgtc ccggtgcagc
      241 aggatggccg ggtggtcggc ttccggctcc acgcaaaact cgacgccatg cggccagaac
      301 ttgtggaagt gcgccttgat ctgcggcaga ggctggccat cgagcggcag gacttcttcc
      361 aggtggctgc ggacacgctc caggtatacg tcttcaccaa gctccaggct gtcccgccag
      421 taggtggcgc ttttgctgtc ggtgtagaac agcacgtatt tgtcgctctt gaagtagatc
      481 ttgcgcaggg gatttgccgc catcagcacc ttgtcggtca gccccagcgc atcccaccag
      541 gctgtgtcga acgtgaagaa tcccttgaac aggggcagcg agtcgtattg gaacggactc
      601 caggcgttcg ggaaatccag gttcaggcgc ggcatggcgg acggcgggat ggccatcacc
      661 agatggcgcg tgcggtgcat ctgcgtgtcg ccatggtggc tgaaggcgag cacatggtcg
      721 gcgcccgatt tttcaacgga cagcaagcgg tgttcgagcc tgaattccac cccggcgacc
      781 tgggcctggt gctgcaactg gcacagcaat tcgtggtagc cgtcggtggc gtagtgccac
      841 tggttggcgg cgttttccgt aaagtgctgc gtttccgggt gcttcttgat gatgtcgtag
      901 gccatggccg ccga
//
