LOCUS       sequence-138192-        8793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Unc5c with a C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: pTT3 Unc5C-FLAG
FEATURES             Location/Qualifiers
     source          1..8793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2814..2837
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2888..2943
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2942..2961)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5179..5197)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5265..5369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5370..5438,5439..6230)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5588..5607)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6401..6989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6890..6909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7143..7160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7277..7298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7313..7318,7319..7336,7337..7343)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7351..7367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7356..7378
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7375..7391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7375..7391
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7662..8041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8042..8245
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8196..8216
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8242..8266
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     8747..8766
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcggccgcgc caccatgagg aaaggtctga gggcgacagc ggcccgctgc ggactgggac
       61 taggatactt gctgcagatg cttgtgttac ctgccctggc cctgctaagc gccagtggca
      121 ccggctccgc cgctcaagat gatgaatttt ttcacgaact cccagaaacc tttccatctg
      181 acccacctga gccattgcca cacttcctca ttgagcccga ggaagcttac attgtgaaga
      241 acaagcctgt gaacctgtat tgtaaagcca gccctgccac ccagatctac ttcaagtgca
      301 acagcgagtg ggttcatcag aaggaccacg tagtagacga gagagtagat gaaacctctg
      361 gtctaattgt gagagaagtg agcattgaga tttcacgcca gcaggtggag gaactgtttg
      421 ggcctgaaga ttactggtgc cagtgtgtgg cctggagctc agcaggcact acgaagagtc
      481 ggaaggcata cgtgcgcatt gcgtatctgc ggaagacatt cgagcaggaa cccttgggaa
      541 aggaagtgtc cttggagcag gaagtcttac tccagtgtcg gccacctgaa gggatcccag
      601 tggctgaggt ggaatggcta aagaatgaag acataattga tcctgctgaa gatcggaact
      661 tttatattac tatcgatcac aacctgatca tcaagcaagc ccgactctca gatacagcaa
      721 attatacctg tgttgccaaa aatattgttg ccaagagaaa aagcaccaca gccactgtca
      781 tcgtgtatgt taatggtggc tggtccacct ggacagagtg gtctgtgtgt aacagccgct
      841 gtgggcgagg atatcagaaa cgcacaagaa cctgcaccaa cccagcccca ctcaatggtg
      901 gggccttctg tgaggggcag agtgtgcaga aaatagcatg cactacgtta tgtccagtgg
      961 atggtaggtg gacttcatgg agcaaatggt caacctgtgg gactgaatgc acccactggc
     1021 gcaggaggga gtgtacagca ccagccccca agaacggggg taaggactgt gatggcctgg
     1081 tcctccaatc caagaactgc actgatgggc tgtgcatgca ggctgctcct gactcagatg
     1141 atgtggctct ctacgtgggg attgtgatcg ctgtaacagt ctgtctggcg atcactgttg
     1201 tggtggccct gtttgtgtat cggaagaacc accgtgactt tgagtctgac atcattgact
     1261 cctcagcact caatggcggc tttcagcctg tgaacatcaa ggctgccaga caagatctcc
     1321 tggctgtccc ccctgacctc acctcagctg cagccatgta caggggacct gtctatgctc
     1381 tgcatgatgt ctcagacaaa atcccaatga ccaactctcc aattctggac ccactaccca
     1441 acttgaaaat caaagtgtac aacagctcag gtgctgtcac tcctcaggat gaccttgccg
     1501 agttctcatc caaactgtca ccccagatga cccagtcctt gctagagaat gaggccctta
     1561 acctgaagaa ccagagcctc gcaagacaga ctgacccatc ctgcacagca tttggtacct
     1621 tcaactctct tgggggtcac ctcatcattc ctaattcagg agtaagcttg ctgattcccg
     1681 ctggggccat tcctcagggg agagtctatg aaatgtatgt gactgtacac aggaaagaaa
     1741 atatgaggcc ccccatggaa gactctcaga ccctacttac ccctgtggtg agctgtgggc
     1801 ctcctggagc tctgctgacc cgccctgtca tcctcactct gcatcactgt gcagacccca
     1861 gcaccgagga ctggaagatc cagctcaaaa accaggcagt gcagggacaa tgggaggatg
     1921 ttgtggtggt tggggaggag aacttcacaa ccccctgtta cattcagctg gatgcagagg
     1981 cttgccatat cctcacagag aacctcagta cctatgccct ggttgggcag tccaccacca
     2041 aagcagctgc caagcgtctt aaactggcca tctttgggcc cctctgctgc tcttccctgg
     2101 agtacagcat tagagtctac tgcctggatg acacacagga tgccctgaag gaagttctac
     2161 aactggagag gcaaatggga ggacagctcc tagaagaacc caaggctctt cattttaaag
     2221 gcagcatcca caacctgcgc ctgtctattc atgacatcgc ccattccctc tggaagagca
     2281 aattgctggc taagtatcag gaaattccat tttaccacat ctggagtggc tctcaaagaa
     2341 acctccactg caccttcact ctggaaagac tcagcctaaa cacagtggaa ctggtttgca
     2401 aactctgtgt gcggcaggtt gaaggagaag ggcagatctt ccagctcaac tgtactgtgt
     2461 cagaggaacc tactggcatc gacttacctc tcctggaccc tgctagtacc atcaccactg
     2521 tcaccggacc aagtgctttc agcattcctc tccctatccg gcagaagcta tgcagcagcc
     2581 tggatgcccc tcaaacaaga ggccatgact ggaggatgct ggcccataaa ctcaacctgg
     2641 acaggtactt gaattacttt gccaccaaat cgagcccaac tggcgtaatc ctggatcttt
     2701 gggaagcaca gaacttccca gatggaaacc tgagcatgct ggcagccgtc ctggaagaaa
     2761 tgggaagaca tgagacagtg gtgtccttgg cagcagaagg acagtatggc ggcgattaca
     2821 aggatgatga tgataagtaa tctagacacg tgattaatta aggatccccc gacctcgacc
     2881 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     2941 tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg gagatctcta
     3001 gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt
     3061 cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     3121 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     3181 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     3241 tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     3301 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     3361 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     3421 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     3481 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     3541 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     3601 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     3661 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     3721 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     3781 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     3841 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     3901 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     3961 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     4021 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     4081 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     4141 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     4201 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     4261 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     4321 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     4381 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     4441 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     4501 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     4561 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     4621 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     4681 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     4741 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     4801 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     4861 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     4921 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     4981 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     5041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     5101 tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa acatgagaat
     5161 tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5221 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5581 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     5641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5881 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     5941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7201 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7261 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7321 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7381 acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc tcatgtttga
     7441 cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta
     7501 ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
     7561 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     7621 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
     7681 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     7741 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     7801 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     7861 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     7921 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     7981 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     8041 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
     8101 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     8161 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
     8221 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
     8281 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
     8341 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
     8401 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
     8461 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
     8521 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
     8581 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     8641 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     8701 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     8761 acaggtgtcc actcccaggt ccaagtttaa act
//
