LOCUS       sequence-138199-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid 
            desaturase domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK125
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgactgacg cgtgtcaagc agagtacgct
      121 atcgcagcta tgtcgaccgt cgagatggac caggcggctc cagagtccgc cgcgcaccac
      181 cctctgccgg accccggtga gtcggtcccc agactcgcgc tgcccacgat cgggatcttc
      241 ctggccacgc tcaccgcgtt cgtcggttct acgaccgctt acatcagcgg atggatcccg
      301 ttctgggtga cgatccccgt caacgccgcg gtcacgttcg tgatgttcac cgtcgtgcat
      361 gacgcatcgc attacgcgat cagctccatc cggtgggtga acgggctgtt cgggcggctg
      421 gcgtggcttt tcgtcgggcc ggtggtcgcg ttcccggcct tcgggtacat ccacatccag
      481 caccaccgcc attccaacga cgacgagcaa gacccggaca ccttcgcctc acacggctcg
      541 ctgtgggtgc tgccgttgcg ctggtcgatg gtcgagtact tctacatcaa gtactacctg
      601 cctcgcggcc gcagccggcc ggtcatcgag gtcgccgaga cgctggtgat gatgaccctg
      661 ttcctgaccg gcctgatcgt cgccatcgtc accggcaact tctggacgct ggcgatcgtc
      721 ttcctgatcc cgcaacgtat cggccttacc gtgctggcct ggtggttcga ctggctgccc
      781 caccacggtc tggaggacac ccagcgcagc aaccgctacc gcgcgacccg caaccgggtt
      841 ggcgccgagt ggctgttcac cccggtgctg ctgtcgcaga actaccactt ggtgcaccac
      901 ctgcacccgt cggtgccgtt ctaccggtac ctgcgcacct ggcggcgcaa cgaggaggcg
      961 tatctggaac gcaacgccgc gatctccacg gtctttggcc agcaactgaa tccggacgag
     1021 taccggcagt ggaaggag
//
