LOCUS       sequence-138201-        8835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Unc5b with a C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: pTT3 Unc5B-FLAG
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2856..2879
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2930..2985
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2984..3003)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5221..5239)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5307..5411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5412..5480,5481..6272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5630..5649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6443..7031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6932..6951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7185..7202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7319..7340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7355..7360,7361..7378,7379..7385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7393..7409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7398..7420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7417..7433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7417..7433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7704..8083
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8084..8287
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8238..8258
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8284..8308
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     8789..8808
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcggccgcgc caccatgagg gcccggagcg gggtgcggag cgcgctgctg ctggcgctgc
       61 tgctttgctg ggatccgaca ccgagcctag caggcgttga ctctgctggc caggtgctcc
      121 cagactccta cccatcagcc cctgcggagc agctgccgta cttcctattg gagccacagg
      181 acgcctacat cgtaaagaac aagccagtgg aactgcactg cagagccttc cctgccacgc
      241 agatctactt caagtgtaat ggcgagtggg tcagccagaa tgaccacgtc acacaggaga
      301 gcctggatga ggccacaggc ttgcgggtgc gagaggtgca gatcgaggtg tcacggcagc
      361 aagtggagga actcttcggg ctcgaggact actggtgcca gtgcgtggcc tggagctctt
      421 cgggaactac caagagtcgc cgagcctaca tccgcattgc ctacttgcgc aagaactttg
      481 accaggagcc tctggccaag gaggtaccct tggatcatga ggtccttctg cagtgccgcc
      541 caccggaggg agtgcctgtg gctgaggtgg aatggctcaa gaatgaagat gtcattgacc
      601 ccgctcagga cactaacttc ctgctcacca ttgaccacaa cctcatcatc cgccaggcgc
      661 gcctctcaga cacggccaac tacacctgtg tggccaagaa tatcgtggcc aagcgccgga
      721 gcaccacggc cacagtcatc gtctatgtga atggaggctg gtccagctgg gcagagtggt
      781 caccctgttc caatcgctgt ggccgaggct ggcagaagcg tactcggacc tgcaccaatc
      841 cagccccact caatggaggc gccttctgtg agggacaggc cttccagaag acagcttgca
      901 ccaccgtgtg cccagtggat ggagcgtgga ccgagtggag caagtggtct gcctgcagca
      961 cagagtgtgc gcactggcgc agccgcgagt gcatggcacc gccaccccag aacggaggcc
     1021 gtgactgcag cgggacgcta cttgactcca agaactgcac tgatgggctg tgcgtgctga
     1081 atcagagaac tctaaacgac cctaaaagcc accccctgga gacatcggga gatgtggcac
     1141 tgtacgcagg ccttgtggtg gccgtctttg tggtggtagc ggttctcatg gccgtgggag
     1201 tgatcgtata ccggagaaac tgccgggact tcgacacgga catcaccgac tcctctgcgg
     1261 ccctcactgg tggcttccac cctgtcaact tcaagactgc aaggcccaac aacccgcagc
     1321 tcctgcaccc gtccgcccct ccagacctaa cggccagtgc tggcatctac cgcgggcctg
     1381 tgtatgccct gcaggactcc gccgacaaga tccccatgac taattcgccc ctgctggatc
     1441 ccctgcccag cctcaagatc aaggtctata actccagcac catcggttct gggtctggcc
     1501 tggctgatgg agccgacctg ctgggtgtcc tcccgccggg cacgtaccca ggcgatttct
     1561 cccgggacac ccatttcctg cacctgcgca gtgccagcct tggttcccag cacctcctgg
     1621 gcctacctcg ggaccccagc agcagtgtca gcggcacctt tggttgcctg ggaggaaggc
     1681 tgagcctccc cggcacaggg gtcagcctgt tggtaccaaa tggagccatt ccccagggca
     1741 agttctatga cctgtatcta catatcaaca aggccgaaag caccctccca ctttcagaag
     1801 gttcccagac agtattgagc ccctcggtga cctgtgggcc cacaggccta ctcctgtgcc
     1861 gccctgtcgt cctcaccgtg ccccactgtg ctgaagtcat cgctggagac tggatctttc
     1921 agctcaagac ccaggcccat cagggccact gggaggaggt ggtgaccttg gatgaggaga
     1981 ccctcaacac accctgctac tgccagctgg aggctaagtc ctgccacatc ctgctggacc
     2041 agctgggtac ctacgtattc atgggcgagt cctactctcg ctctgcagtc aagcggctcc
     2101 agctggccat cttcgcccca gccctctgca cctccctgga gtatagcctc agggtctact
     2161 gtctggagga cacacctgta gcactgaagg aggtcctgga gctggagagg actctgggtg
     2221 gctacttggt ggaggagccc aagcctttgc tctttaagga cagttaccac aacctacgcc
     2281 tctccctcca tgacatcccc catgcccact ggaggagcaa actactggcc aagtaccagg
     2341 agattccctt ctaccacgtc tggaatggca gccagagagc cctgcactgc actttcaccc
     2401 tggagaggca tagcctggcc tccacggagt tcacctgtaa ggtctgcgtg cggcaggtcg
     2461 aaggggaagg ccagattttc cagctgcaca caacgttggc cgagacgcct gctggctccc
     2521 tggatgctct ctgctctgcc ccgggcaatg ccatcaccac ccagctggga ccctatgcct
     2581 tcaagatacc cctgtccatc cgccaaaaga tctgcagcag cctggacgcc cccaactccc
     2641 ggggcaacga ctggaggctg ttggcgcaga agctgtccat ggaccggtac ctaaactact
     2701 tcgccaccaa agctagtccc acaggtgtca tcttagacct ctgggaagct cggcaacagg
     2761 atgacgggga cctcaacagc ctggccagtg ccttggagga gatgggcaag agtgagatgc
     2821 tggtagccat ggctacagat ggcgattgcg gcggcgatta caaggatgat gatgataagt
     2881 aatctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa
     2941 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     3001 gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc
     3061 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     3121 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
     3181 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
     3241 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
     3301 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
     3361 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
     3421 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
     3481 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
     3541 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
     3601 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
     3661 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
     3721 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
     3781 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
     3841 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
     3901 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
     3961 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
     4021 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
     4081 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
     4141 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
     4201 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
     4261 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
     4321 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
     4381 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
     4441 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
     4501 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
     4561 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
     4621 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     4681 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     4741 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4801 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt
     4861 agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga
     4921 tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     4981 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     5041 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     5101 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     5161 tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg
     5221 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5641 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5941 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7081 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7141 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7201 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7321 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7381 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7441 acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat
     7501 ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac
     7561 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
     7621 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     7681 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     7741 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     7801 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     7861 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     7921 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
     7981 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
     8041 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     8101 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     8161 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     8221 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     8281 cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg tctgcgaggg
     8341 ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt actcttggat
     8401 cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc gagtccgcat
     8461 cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc
     8521 tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg gaggtgctgc
     8581 tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg aggtgtggca
     8641 ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat tacttctgcg
     8701 ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc cgatctggcc
     8761 atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag
     8821 gtccaagttt aaact
//
