LOCUS       sequence-138204-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 citrate synthase with cleavable 
            N-terminal His6/CBD/Ssp-intein fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK237
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             115..267
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVP
                     ALWQLQ"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaaaatcg aagaaggtaa actgacaaat
      121 cctggtgtat ccgcttggca ggtcaacaca gcttatactg cgggacaatt ggtcacatat
      181 aacggcaaga cgtataaatg tttgcagccc cacacctcct tggcaggatg ggaaccatcc
      241 aacgttcctg ccttgtggca gcttcaaaac aacggtaaca acggtctcga actgcgcgag
      301 tccggagcta tctctggcga tagtctgatc agcctggcta gcacaggaaa aagagtttct
      361 attaaagatt tgttagatga aaaagatttt gaaatatggg caattaatga acagacgatg
      421 aagctagaat cagctaaagt tagtcgtgta ttttgtactg gcaaaaagct agtttatatt
      481 ctaaaaactc gactaggtag aactatcaag gcaacagcaa atcatagatt tttaactatt
      541 gatggttgga aaagattaga tgagctatct ttaaaagagc atattgctct accccgtaaa
      601 ctagaaagct cctctttaca attgtcacca gaaatagaaa agttgtctca gagtgatatt
      661 tactgggact ccatcgtttc tattacggag actggagtcg aagaggtttt tgatttgact
      721 gtgccaggac cacataactt tgtcgcgaat gacatcattg tacacaacat gagcgcgtcg
      781 cagaaagaag gtaagctatc taccgctacc atttcggttg atggaaaatc cgccgaaatg
      841 cctgtgcttt caggcactct gggaccggat gttatcgaca tccgcaaact tccggcgcaa
      901 ctgggcgttt tcacgttcga cccaggttac ggggaaacag cggcctgcaa cagcaaaatc
      961 acctttattg atggtgataa aggcgttctg ctgcaccgtg gttaccc
//
