LOCUS       sequence-138206-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 citrate synthase with cleavable 
            N-terminal His6/CBD/Ssp-intein fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK237
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattct tagcgtttgg caagcggcac atagtcacgc tgcggtgcgc
      121 caatataaag ctggcgaggg cggctgatac gctggcccgg ttcttcaatc atttccttcc
      181 actggctcac ccagccggtg gtgcgggcta cggcaaacag cacagtaaac atactggtgg
      241 ggatgcccat ggccttgaga atgatgccag agtagaaatc cacattcggg taaagtttgc
      301 gctgcacgaa gtaatcatcg ctcagagcaa tcttttccag ctcaaccgcc agatccagca
      361 gcggatcatc cttaatgcca agttctgtca gcacttcgtg gcaggtctgc tgcatgatct
      421 tcgcacgtgg gtcgaagttc ttgtaaacgc ggtggccaaa gcccatcagc tttacgccgc
      481 tgttcttgtc cttcacctgt gcgataaagg caggaatatt ttctttcttg ccaatacggg
      541 ccagcatttt cagcacagct tcgtttgcgc caccatgtgc aggtccccac agagcggcaa
      601 tgcccgcagc aatacaggca aacggattgg cccctgtaga accagccaga cgtacggtgg
      661 aggtagaggc attctgctca tgatcggcat gcagaatcag aatccggttc atggcgcggg
      721 ccagaacagg gttgaccttg taaggttcgg acatgcgcgc gaacatcatg gacaggaagt
      781 tttctgcgta gttcagatca ttccgcgggt agataaaggc ttcaccctgc gtgtatttgt
      841 aagcccatgc cgcaatggtt gggattttgg caatcagccg catggcggcc agatcccgat
      901 tggcgggaat ggcaa
//
