LOCUS       sequence-138211-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/spoR
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             38..58
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             735..752
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aggagatata catatgcatc atcatcatca tcacggcgaa aacctgtatt ttcagggcgg
       61 atccggatcc atggctcaag gcacgcttta tattgtttct gcccccagtg gcgcgggtaa
      121 atccagcctg attcaggctt tattaaaaac ccaaccgttg tatgacaccc aagtttctgt
      181 ttcacacacc acacgccaac cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt
      241 taatcatgat gaatttaaag aaatgattag cagagatgcg ttcctcgaac acgcagaagt
      301 ttttggtaat tactatggca cttcgcgtga ggccattgag caagtactgg cgaccggtgt
      361 cgatgttttt ctcgatatcg actggcaggg cgcgcagcaa attcgccaga agatgccgca
      421 cgcgcggagt atctttattt taccgccgtc caaaattgaa ctggaccgcc gtctacgcgg
      481 tcgcggtcag gacagcgaag aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat
      541 gagccattac gccgaatatg attatctgat tgtgaatgat gacttcgata ccgcgttgac
      601 cgatttgaag accattattc gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca
      661 tgacgcttta atcagcaaat tgttggcaga ctgagaattc gagctccgtc gacaagcttg
      721 cggccgcact cgagcaccac caccaccacc actg
//
