LOCUS       sequence-138214-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide 
            ribonucleotide synthetase (PurC), N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/purC
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac atatgcatca tcatcatcat cacggcgaaa acctgtattt
       61 tcagggcgga tccatgcaaa agcaagctga gttgtatcgt ggtaaagcga aaaccgtata
      121 cagcacggaa aacccggacc tgttggtgct cgaattccgc aatgatacgt cagcagggga
      181 tggcgcgcgc attgagcagt ttgatcgcaa aggtatggtg aacaacaagt tcaactactt
      241 cattatgagc aaactggctg aagcgggtat cccgactcaa atggagcgtc tgctctccga
      301 taccgaatgt ctggtgaaaa agctggatat ggtgccggtt gagtgtgtcg tgcgtaaccg
      361 tgctgctggc tctctggtga aacgtcttgg aatcgaagaa ggtattgagc tgaacccgcc
      421 gctgttcgat ctgttcctga aaaacgacgc catgcacgat ccgatggtca acgaatctta
      481 ctgcgaaacc tttggctggg tgagcaaaga gaacctggcg cgtatgaaag agctgaccta
      541 caaagcgaac gacgtgctga aaaaactgtt cgatgatgct ggtctgattc tggtcgactt
      601 caagctggag tttggtctgt acaaaggtga agtggtactg ggcgatgagt tctccccgga
      661 cggcagccgc ctgtgggaca aagaaacact ggagaaaatg gacaaagacc gtttccgcca
      721 ga
//
