LOCUS       sequence-138219-        9119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neuropilin 2, isoform 1 
            protein, C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Nrp2.1-AP-His
FEATURES             Location/Qualifiers
     source          1..9119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5699..5716
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5742..5863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5779..5798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5833..5852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5901..5918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6072..6660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6152..6171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6831..7622,7623..7691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7454..7473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7692..7796)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7864..7882
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7920..7942)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7944..7964)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7949..8279
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8130..8265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8192..8211
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8416..8435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8502..8957)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8639..8660)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8851..8870
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9083..9105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9097..9114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9098..9114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggatatgtt
     1621 tcctcttacc tgggttttct tagctctgta cttttcagga cacgaagtga gaagccagca
     1681 agatccaccc tgcggaggtc ggctgaattc caaagatgct ggctacatca cttccccagg
     1741 ctacccccag gactatccct cccaccagaa ctgtgagtgg attgtctacg cccccgaacc
     1801 caaccagaag attgttctca acttcaaccc tcactttgaa atcgagaaac acgactgcaa
     1861 gtatgacttc attgagattc gggatgggga cagtgagtca gctgacctcc tgggcaagca
     1921 ctgtgggaac atcgccccgc ccaccatcat ctcctcaggc tccgtgttat acatcaagtt
     1981 cacctcagac tacgcccggc agggggcagg tttctctcta cgctatgaga tcttcaaaac
     2041 aggctctgaa gattgttcca agaactttac aagccccaat gggaccattg aatctccagg
     2101 gtttccagag aagtatccac acaatctgga ctgtaccttc accatcctgg ccaaacccag
     2161 gatggagatc atcctacagt tcctgacctt tgacctggag catgaccctc tacaagtggg
     2221 ggaaggagac tgtaaatatg actggctgga catctgggat ggcattccac atgttggacc
     2281 tctgattggc aagtactgtg ggacgaaaac accctccaaa ctccgctcgt ccacggggat
     2341 cctctccttg acctttcaca cggacatggc agtggccaag gatggcttct ccgcacgtta
     2401 ctatttgatc caccaggagc cacctgagaa ttttcagtgc aatgtccctt tgggaatgga
     2461 gtctggccgg attgctaatg aacagatcag tgcctcctcc accttctctg atgggaggtg
     2521 gactcctcaa cagagccggc tccatggtga tgacaatggc tggacaccca atttggattc
     2581 caacaaggag tatctccagg tggacctgcg cttcctaacc atgctcacag ccattgcaac
     2641 acagggagcc atttccaggg aaacccagaa aggctactac gtcaaatcgt acaagctgga
     2701 agtcagcaca aatggtgaag attggatggt ctaccggcat ggcaaaaacc acaagatatt
     2761 ccaagcgaac aatgatgcga ccgaggtggt gctaaacaag ctccacatgc cactgctgac
     2821 tcggttcatc aggatccgcc cgcagacgtg gcatttgggc attgcccttc gcctggagct
     2881 ctttggctgc cgggtcacag atgcaccctg ctccaacatg ctggggatgc tctcgggcct
     2941 cattgctgat acccagatct ctgcctcctc cacccgagag tacctctgga gccccagtgc
     3001 tgcccgcctg gttagtagcc gctctggctg gtttcctcgg aaccctcaag cccagccagg
     3061 tgaagaatgg cttcaggtag acctggggac acccaagaca gtgaaagggg tcatcatcca
     3121 gggagcccga ggaggagaca gcatcactgc cgtggaagcc agggcgtttg tacgcaagtt
     3181 caaagtctcc tacagcctaa atggcaagga ctgggaatat atccaggacc ccaggactca
     3241 gcagacaaag ctgtttgaag ggaacatgca ctatgacacc cctgacatcc gaaggttcga
     3301 tcctgttcca gcgcagtatg tgcgggtgta cccagagagg tggtcgccag caggcatcgg
     3361 gatgaggctg gaggtgctgg gctgtgactg gacagactca aagcccacag tggagacgct
     3421 gggacccacc gtgaagagtg aagagactac caccccatat cccatggatg aggatgccac
     3481 cgagtgtggg gaaaactgca gctttgagga tgacaaagat ttgcaacttc cttcaggatt
     3541 caactgcaac tttgattttc cggaagagac ctgtggttgg gtgtacgacc atgccaagtg
     3601 gctccggagc acgtggatca gcagcgctaa ccccaatgac agaacatttc cagatgacaa
     3661 gaacttcttg aaactgcaga gtgatggccg acgagagggc cagtacgggc ggctcatcag
     3721 cccaccggtg cacctgcccc gaagccctgt gtgcatggag ttccagtacc aagccatggg
     3781 cggccacggg gtggcactgc aggtggttcg ggaagccagc caggaaagca aactcctttg
     3841 ggtcatccgt gaggaccagg gcagcgagtg gaaacacggg cgcattatcc tgcccagcta
     3901 tgacatggag tatcagatcg tgttcgaggg agtgataggg aagggacgat cgggagagat
     3961 ttccatcgat gacattcgga taagcactga tgtcccactg gagaactgca tggaacccat
     4021 atcagctttt gcaggtgagg attttaaagt ggacatccca gaaacccatg ggggagaggg
     4081 ctatgaagat gagattgatg atgaatatga aggagattgg agcaactctt cttcctctac
     4141 ctcaggggct ggtgacccct catctggcaa agaaaagagc tggctgtaca ccctagatcc
     4201 cattctgatc ggcgcgcctg gatcttccgg aatcatccca gttgaggagg agaacccgga
     4261 cttctggaac cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca
     4321 gacagccgcc aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac
     4381 agctgccagg atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc
     4441 catggaccgc ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc
     4501 agacagtgga gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat
     4561 tggcttgagt gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat
     4621 ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg
     4681 agtgcagcac gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc
     4741 ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct
     4801 catctccaac atggacattg acgtgatcct aggtgggggc cgaaagtaca tgtttcgcat
     4861 gggaacccca gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg
     4921 gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg
     4981 cactgagctc atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga
     5041 gcctggagac atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga
     5101 gatgacagag gctgccctgc gcctgctgag caggaacccc cgcggcttct tcctcttcgt
     5161 ggagggtggt cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga
     5221 gacgatcatg ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac
     5281 gctgagcctc gtcactgccg accactccca cgtcttctcc ttcggaggct accccctgcg
     5341 agggagctcc atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt
     5401 cctcctatac ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac
     5461 cgagagcgag agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga
     5521 gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca
     5581 cggcgtgcag gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc
     5641 ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtca
     5701 tcatcaccat caccattaat ctagagatct tattaaagca gaacttgttt attgcagctt
     5761 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5821 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggtcgactc
     5881 tagctagact cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6481 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7141 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7441 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7801 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7861 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     7921 ctctgacaca tgcagctccc ggaggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7981 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     8041 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8101 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8161 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     8221 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8281 agctcccggg agcttgtata tccattttcg tcccggagac ggtcacagct tgtctgtaag
     8341 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     8401 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     8461 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     8521 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8581 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8641 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     8701 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     8761 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     8821 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     8881 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     8941 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     9001 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     9061 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatt
//
