LOCUS       sequence-138222-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/spoR
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(709..729)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(733..750)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tctcagtctg ccaacaattt gctgattaaa gcgtcatgac gctgcttttg
      121 gcggctcatg cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca acgcggtatc
      181 gaagtcatca ttcacaatca gataatcata ttcggcgtaa tggctcattt ctgcaacagc
      241 ttgcgccata cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg
      301 gtccagttca attttggacg gcggtaaaat aaagatactc cgcgcgtgcg gcatcttctg
      361 gcgaatttgc tgcgcgccct gccagtcgat atcgagaaaa acatcgacac cggtcgccag
      421 tacttgctca atggcctcac gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc
      481 gaggaacgca tctctgctaa tcatttcttt aaattcatca tgattaacaa agaaataatg
      541 ttcaccgtgg acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag aaacttgggt
      601 gtcatacaac ggttgggttt ttaataaagc ctgaatcagg ctggatttac ccgcgccact
      661 gggggcagaa acaatataaa gcgtgccttg agccatggat ccggatccgc cctgaaaata
      721 caggttttcg ccgtgatgat gatgatgatg catatgtata tctccttctt aa
//
