LOCUS       sequence-138223-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide 
            ribonucleotide synthetase (PurC), N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/purC
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtc agtccagctg tacacccagg
       61 cggcgggcga cggcttcata ggcctcgatc aggccaccga ggctctggcg gaaacggtct
      121 ttgtccattt tctccagtgt ttctttgtcc cacaggcggc tgccgtccgg ggagaactca
      181 tcgcccagta ccacttcacc tttgtacaga ccaaactcca gcttgaagtc gaccagaatc
      241 agaccagcat catcgaacag ttttttcagc acgtcgttcg ctttgtaggt cagctctttc
      301 atacgcgcca ggttctcttt gctcacccag ccaaaggttt cgcagtaaga ttcgttgacc
      361 atcggatcgt gcatggcgtc gtttttcagg aacagatcga acagcggcgg gttcagctca
      421 ataccttctt cgattccaag acgtttcacc agagagccag cagcacggtt acgcacgaca
      481 cactcaaccg gcaccatatc cagctttttc accagacatt cggtatcgga gagcagacgc
      541 tccatttgag tcgggatacc cgcttcagcc agtttgctca taatgaagta gttgaacttg
      601 ttgttcacca tacctttgcg atcaaactgc tcaatgcgcg cgccatcccc tgctgacgta
      661 tcattgcgga attcgagcac caacaggtcc gggttttccg tgctgtatac ggttttcgct
      721 ttaccacgat acaactcagc ttgcttttgc atggatccgc cctgaa
//
