LOCUS       sequence-138226-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID-mClover using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK291 (mAID-mClover-Bsr)
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg
       61 aagcttaccg ctagcatgaa aacatttaac atttctcaac aagatctaga attagtagaa
      121 gtagcgacag agaagattac aatgctttat gaggataata aacatcatgt gggagcggca
      181 attcgtacga aaacaggaga aatcatttcg gcagtacata ttgaagcgta tataggacga
      241 gtaactgttt gtgcagaagc cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat
      301 tttgacacga ttgtagctgt tagacaccct tattctgacg aagtagatag aagtattcga
      361 gtggtaagtc cttgtggtat gtgtagggag ttgatttcag actatgcacc agattgtttt
      421 gtgttaatag aaatgaatgg caagttagtc aaaactacga ttgaagaact cattccactc
      481 aaatataccc gaaattaagc tagccgcccg ccccacgacc cgcagcgccc gaccgaaagg
      541 agcgcacgac cccatgcatc gatgatatca gatccccggg atgcagaaat tgatgatcta
      601 ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt gttaagaagg
      661 gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg
      721 gtgggatt
//
