LOCUS       sequence-138237-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OsTIR1 under the control of the Tet promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK243 (Tet-OsTIR1-PURO)
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(172..918)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
       61 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
      121 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctggaga tttacccggg
      181 gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc
      241 gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc
      301 gccgctttcg cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag
      361 gaaggcaggt tcggctccct gccggtcgaa cagctcaatt gcttgtttca gaagtggggg
      421 catagaatcg gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc
      481 caatacgcag cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag
      541 ggagaagcct tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc
      601 tgttgggcgg gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg
      661 gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagga
      721 gtgggtatga tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt
      781 cttcacgtgc cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt
      841 cgtcaggcct tcgataccga ctccattgag taattccaga gcagagttta tgactttgct
      901 cttgtccagt ctagacatgg taattcgatg atccccctgg ggagagaggt cggtgattcg
      961 gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc ttgca
//
