LOCUS       sequence-138238-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Janthinobacterium lividum violacein biosynthesis protein 
            VioC with a N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Kappock
  TITLE     Janthinobacterium lividum violacein biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK265
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgcataaaa tcattatcgt cggcggaggc
      121 ctggcaggca gcctcagcgc catttatctg gcgcaacggg ggcacgatgt ccacgttgtc
      181 gaaaagcgcg gcgatccgct gctggagaat gccgcaaacg ccgaccccgt caactcgcgc
      241 gccatcggcg tgagcatgac ggtacgcggc atcaaggccg tcctggccgc cggcatcagc
      301 aagcaggagc tcgaccagtg cggcgaaccc atcgtcggca tggcattcag cgtgggcggc
      361 cggcaccgga tacgcgagct gaccccgctc gaaggcctgt tccccctgtc gctggaccgc
      421 accgccttcc agcgcctgct gaaccggcat gccgccctgc acgaggtgaa gtattacttt
      481 gagcataaat gcctggatgt cgacctggaa agaaagatcg tgctgatcca gggcccggac
      541 ggcgccttgc agaagctgca tggcgacctg gtcattggcg ccgacggcgc ccactctgcc
      601 gtgcggcgcg ccatgcaaag cggcatgcgc cgtttcgagt tcaggcaaag ttacttccgc
      661 cacggctaca agacgctggt gttgccgaac gcggcggatc tgggtttcag gaaggatttg
      721 ctgtacttct tcggcatgga ttccaagggc ctgtttgccg gccgcgcggc caccatcccg
      781 gacggcagca tcagctttgc cctgtgcctg ccctacaccg gcacgcccag cctgggcacg
      841 ctcaaccgcg aagccatggc cgatttcttc agccgctact tcggcaccct gccgccggac
      901 cgtcgcaagg aaatgctgga ccagttcatg gcgctgccca gcaacgacct catcaatgtc
      961 cgttccagca ccttccacta caaggccaat atcctgctga tcggcgatgc ggcgcatgcc
     1021 accg
//
