LOCUS       sequence-138251-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK288 (mAID-Bsr)
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct agatctcgag
       61 cagctgaagc ttaccgctag catgaaaaca tttaacattt ctcaacaaga tctagaatta
      121 gtagaagtag cgacagagaa gattacaatg ctttatgagg ataataaaca tcatgtggga
      181 gcggcaattc gtacgaaaac aggagaaatc atttcggcag tacatattga agcgtatata
      241 ggacgagtaa ctgtttgtgc agaagccatt gcgattggta gtgcagtttc gaatggacaa
      301 aaggattttg acacgattgt agctgttaga cacccttatt ctgacgaagt agatagaagt
      361 attcgagtgg taagtccttg tggtatgtgt agggagttga tttcagacta tgcaccagat
      421 tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa ctacgattga agaactcatt
      481 ccactcaaat atacccgaaa ttaagctagc cgcccgcccc acgacccgca gcgcccgacc
      541 gaaaggagcg cacgacccca tgcatcgatg atatcagatc cccgggatgc agaaattgat
      601 gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta
      661 agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg
      721 ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact attgctttat
      781 gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc
      841 tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct
      901 cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc
      961 tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc
     1021 aagttctaat tccatcagaa gctggtcgag atccggaacc ctta
//
