LOCUS       sequence-138272-        2645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded super-folder GFP in universal C-terminal tagging 
            plasmid, G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS292 C terminal SF-GFP kanR
FEATURES             Location/Qualifiers
     source          1..2645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gene            1251..2607
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1251..1594
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1595..2404
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1662..1681)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2410..2607
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gtttctaagg gtgaagaact ttttactggt
       61 gttgttccta ttcttgttga acttgatggt gatgttaacg gtcataagtt ttctgttcgt
      121 ggtgaaggtg aaggtgatgc tactaatggt aaacttactc ttaagtttat ttgcactact
      181 ggtaagttgc ctgttccttg gcctacttta gttactactc ttacttatgg tgttcaatgc
      241 ttttctcgtt atcctgatca tatgaagcaa catgattttt ttaagtctgc tatgcctgaa
      301 ggttatgttc aagaacgtac tatttctttt aaggatgatg gtacttacaa gactcgtgct
      361 gaagttaagt ttgaaggtga tactcttgtt aatcgtattg aacttaaggg tattgatttt
      421 aaagaagatg gtaatattct tggtcataag cttgaataca actttaattc tcataacgtt
      481 tacattactg ctgataagca aaagaacggt attaaggcta actttaagat tcgtcataat
      541 gttgaagatg gttctgttca acttgctgat cattaccaac aaaacactcc tattggtgat
      601 ggtcctgttc ttcttcctga taaccattac ctttctactc aatctgttct ttctaaggat
      661 cctaacgaaa agcgtgatca tatggttctt ttggaatttg ttactgctgc tggtattact
      721 cttggtatgg atgaacttta caagtaaaaa aagactaatg taaaattttt ttggttggtt
      781 attgaaaaag tcgatgcctt gtttgcgttt gttttcctag gcgttttatg tcagaaggca
      841 tttagaatta gtatacaagt actctttggt aaaattttat gtagcgacta aaatattaac
      901 tattatagat aaacaccttg ggaataaaaa gtaatttgct atagtaattt attaaacatg
      961 ctcctacaac attaccacaa tcttttctct tggattgaca ttgaataaga aaagagtgaa
     1021 tttttttaga cttgtaatga taactatgta caaagccaat gaaagatgta tgtagatgaa
     1081 tgtaaaatac catgtagaca aacaagataa aacttggtta taaacattgg tgttggaaca
     1141 gaataaatta gatgtcaaaa agtttcgtca atatcaccgg atccccgggt taattaaggc
     1201 gcgccagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
     1261 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
     1321 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
     1381 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
     1441 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
     1501 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
     1561 acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc
     1621 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1681 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     1741 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1801 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1861 tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga
     1921 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1981 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2041 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2101 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga
     2161 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2221 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2281 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2341 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2401 ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
     2461 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
     2521 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat
     2581 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt
     2641 taaac
//
