LOCUS       sequence-138273-        2585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS291 C terminal mKO2 kanR
FEATURES             Location/Qualifiers
     source          1..2585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..678
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     gene            1191..2547
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1191..1534
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1535..2344
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1602..1621)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2350..2547
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gtttctgtta ttaagcctga aatgaagatg
       61 cgttactata tggatggttc tgttaatggt catgaattta ctattgaagg tgaaggtact
      121 ggtcgtcctt atgaaggtca tcaagaaatg actcttcgtg ttactatggc tgaaggtggt
      181 cctatgcctt ttgcttttga tcttgtttct catgtttttt gctatggtca tcgtgttttt
      241 actaagtatc ctgaagaaat tcctgattac tttaagcaag cttttcctga aggtctttct
      301 tgggaacgtt ctcttgaatt tgaagatggt ggttctgctt ctgtttctgc tcatatttct
      361 cttcgtggta atacttttta ccataagtct aagtttactg gtgttaattt tcctgctgat
      421 ggtcctatta tgcaaaatca atctgttgat tgggaacctt ctactgaaaa gattactgct
      481 tctgatggtg ttcttaaggg tgatgttaca atgtacctta agttagaagg tggtggtaac
      541 cataagtgcc aaatgaagac tacttacaag gctgctaaag aaattcttga aatgcctggt
      601 gatcattaca ttggacatcg tcttgttcgt aagactgaag gtaacattac tgaacaagtt
      661 gaagatgctg ttgctcatta ttcttaaaaa aagactaatg taaaattttt ttggttggtt
      721 attgaaaaag tcgatgcctt gtttgcgttt gttttcctag gcgttttatg tcagaaggca
      781 tttagaatta gtatacaagt actctttggt aaaattttat gtagcgacta aaatattaac
      841 tattatagat aaacaccttg ggaataaaaa gtaatttgct atagtaattt attaaacatg
      901 ctcctacaac attaccacaa tcttttctct tggattgaca ttgaataaga aaagagtgaa
      961 tttttttaga cttgtaatga taactatgta caaagccaat gaaagatgta tgtagatgaa
     1021 tgtaaaatac catgtagaca aacaagataa aacttggtta taaacattgg tgttggaaca
     1081 gaataaatta gatgtcaaaa agtttcgtca atatcaccgg atccccgggt taattaaggc
     1141 gcgccagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
     1201 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
     1261 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
     1321 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
     1381 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
     1441 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
     1501 acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc
     1561 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1621 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     1681 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1741 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1801 tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga
     1861 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1921 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1981 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2041 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga
     2101 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2161 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2221 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2281 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2341 ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
     2401 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
     2461 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat
     2521 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt
     2581 taaac
//
