LOCUS       sequence-138276-        2776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS285 C terminal mKO2 hygBR
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..678
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     gene            1171..2746
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        1171..1514
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1515..2543
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1515..2543
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2549..2746
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gtttctgtta ttaagcctga aatgaagatg
       61 cgttactata tggatggttc tgttaatggt catgaattta ctattgaagg tgaaggtact
      121 ggtcgtcctt atgaaggtca tcaagaaatg actcttcgtg ttactatggc tgaaggtggt
      181 cctatgcctt ttgcttttga tcttgtttct catgtttttt gctatggtca tcgtgttttt
      241 actaagtatc ctgaagaaat tcctgattac tttaagcaag cttttcctga aggtctttct
      301 tgggaacgtt ctcttgaatt tgaagatggt ggttctgctt ctgtttctgc tcatatttct
      361 cttcgtggta atacttttta ccataagtct aagtttactg gtgttaattt tcctgctgat
      421 ggtcctatta tgcaaaatca atctgttgat tgggaacctt ctactgaaaa gattactgct
      481 tctgatggtg ttcttaaggg tgatgttaca atgtacctta agttagaagg tggtggtaac
      541 cataagtgcc aaatgaagac tacttacaag gctgctaaag aaattcttga aatgcctggt
      601 gatcattaca ttggacatcg tcttgttcgt aagactgaag gtaacattac tgaacaagtt
      661 gaagatgctg ttgctcatta ttcttaaaaa aagactaatg taaaattttt ttggttggtt
      721 attgaaaaag tcgatgcctt gtttgcgttt gttttcctag gcgttttatg tcagaaggca
      781 tttagaatta gtatacaagt actctttggt aaaattttat gtagcgacta aaatattaac
      841 tattatagat aaacaccttg ggaataaaaa gtaatttgct atagtaattt attaaacatg
      901 ctcctacaac attaccacaa tcttttctct tggattgaca ttgaataaga aaagagtgaa
      961 tttttttaga cttgtaatga taactatgta caaagccaat gaaagatgta tgtagatgaa
     1021 tgtaaaatac catgtagaca aacaagataa aacttggtta taaacattgg tgttggaaca
     1081 gaataaatta gatgtcaaaa agtttcgtca atatcacggc gcgccagatc tgtttagctt
     1141 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     1201 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1261 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     1321 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     1381 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     1441 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     1501 gaacataaac aaccatgggt aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     1561 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     1621 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     1681 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     1741 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     1801 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     1861 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     1921 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     1981 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     2041 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     2101 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     2161 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     2221 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     2281 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     2341 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     2401 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     2461 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     2521 ctcgtccgag ggcaaaggaa taatcagtac tgacaataaa aagattcttg ttttcaagaa
     2581 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     2641 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     2701 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     2761 ctaacgccgc catcca
//
