LOCUS       sequence-138277-        2802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            hygB resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS287 C terminal E2C hygBR
FEATURES             Location/Qualifiers
     source          1..2802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gene            1189..2764
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        1189..1532
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1533..2561
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1533..2561
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2567..2764
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gattctactg aaaacgttat taagcctttt
       61 atgcgtttta aggttcatat ggaaggttct gttaatggtc atgaatttga aattgaaggt
      121 gttggtgaag gtaagcctta tgaaggtact caaactgcta agcttcaagt tacaaaaggt
      181 ggtcctttac cttttgcttg ggatattctt tctcctcaat ttttttacgg ttctaaagct
      241 tacattaagc atcctgctga tattccagat taccttaagc aatcatttcc tgaaggtttt
      301 aagtgggaac gtgttatgaa ctttgaagat ggtggtgttg ttactgttac tcaagattct
      361 tcacttcaag atggtactct tatttaccat gttaagttta ttggtgttaa ttttccttct
      421 gatggtcctg ttatgcaaaa aaagacttta ggttgggaac cttctacaga acgtaattat
      481 cctcgtgatg gtgttcttaa gggtgaaaat catatggctc ttaagttgaa aggtggtggt
      541 cattaccttt gcgaatttaa gtctatttac atggctaaga agcctgttaa gcttcctggt
      601 tatcattacg ttgattacaa gcttgatatt acttctcata atgaagatta tactgttgtt
      661 gaacaatatg aacgtgctga agctcgtcat catctttttc aataaaaaaa gactaatgta
      721 aaattttttt ggttggttat tgaaaaagtc gatgccttgt ttgcgtttgt tttcctaggc
      781 gttttatgtc agaaggcatt tagaattagt atacaagtac tctttggtaa aattttatgt
      841 agcgactaaa atattaacta ttatagataa acaccttggg aataaaaagt aatttgctat
      901 agtaatttat taaacatgct cctacaacat taccacaatc ttttctcttg gattgacatt
      961 gaataagaaa agagtgaatt tttttagact tgtaatgata actatgtaca aagccaatga
     1021 aagatgtatg tagatgaatg taaaatacca tgtagacaaa caagataaaa cttggttata
     1081 aacattggtg ttggaacaga ataaattaga tgtcaaaaag tttcgtcaat atcacggcgc
     1141 gccagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     1201 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     1261 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     1321 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     1381 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     1441 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     1501 agttctcaca tcacatccga acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga
     1561 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     1621 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     1681 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     1741 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     1801 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     1861 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     1921 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     1981 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     2041 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     2101 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     2161 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     2221 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     2281 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     2341 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     2401 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     2461 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     2521 gaaaccgacg ccccagcact cgtccgaggg caaaggaata atcagtactg acaataaaaa
     2581 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     2641 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     2701 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     2761 actgctgtcg attcgatact aacgccgcca tccagtttaa ac
//
