LOCUS       sequence-138278-        2603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS289 C terminal E2C G418R
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gene            1209..2565
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1209..1552
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1553..2362
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1620..1639)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2368..2565
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gattctactg aaaacgttat taagcctttt
       61 atgcgtttta aggttcatat ggaaggttct gttaatggtc atgaatttga aattgaaggt
      121 gttggtgaag gtaagcctta tgaaggtact caaactgcta agcttcaagt tacaaaaggt
      181 ggtcctttac cttttgcttg ggatattctt tctcctcaat ttttttacgg ttctaaagct
      241 tacattaagc atcctgctga tattccagat taccttaagc aatcatttcc tgaaggtttt
      301 aagtgggaac gtgttatgaa ctttgaagat ggtggtgttg ttactgttac tcaagattct
      361 tcacttcaag atggtactct tatttaccat gttaagttta ttggtgttaa ttttccttct
      421 gatggtcctg ttatgcaaaa aaagacttta ggttgggaac cttctacaga acgtaattat
      481 cctcgtgatg gtgttcttaa gggtgaaaat catatggctc ttaagttgaa aggtggtggt
      541 cattaccttt gcgaatttaa gtctatttac atggctaaga agcctgttaa gcttcctggt
      601 tatcattacg ttgattacaa gcttgatatt acttctcata atgaagatta tactgttgtt
      661 gaacaatatg aacgtgctga agctcgtcat catctttttc aataaaaaaa gactaatgta
      721 aaattttttt ggttggttat tgaaaaagtc gatgccttgt ttgcgtttgt tttcctaggc
      781 gttttatgtc agaaggcatt tagaattagt atacaagtac tctttggtaa aattttatgt
      841 agcgactaaa atattaacta ttatagataa acaccttggg aataaaaagt aatttgctat
      901 agtaatttat taaacatgct cctacaacat taccacaatc ttttctcttg gattgacatt
      961 gaataagaaa agagtgaatt tttttagact tgtaatgata actatgtaca aagccaatga
     1021 aagatgtatg tagatgaatg taaaatacca tgtagacaaa caagataaaa cttggttata
     1081 aacattggtg ttggaacaga ataaattaga tgtcaaaaag tttcgtcaat atcaccggat
     1141 ccccgggtta attaaggcgc gccagatctg tttagcttgc ctcgtccccg ccgggtcacc
     1201 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
     1261 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
     1321 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
     1381 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
     1441 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
     1501 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
     1561 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1621 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     1681 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1741 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1801 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     1861 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1921 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1981 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2041 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     2101 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2161 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2221 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2281 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2341 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     2401 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     2461 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     2521 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     2581 taacgccgcc atccagttta aac
//
