LOCUS       sequence-138279-        2844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded superfolder GFP in universal C-terminal tagging 
            plasmid, hygB resistanc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS286 C terminal SF-GFP hygBR
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gene            1231..2806
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        1231..1574
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1575..2603
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1575..2603
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2609..2806
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gtttctaagg gtgaagaact ttttactggt
       61 gttgttccta ttcttgttga acttgatggt gatgttaacg gtcataagtt ttctgttcgt
      121 ggtgaaggtg aaggtgatgc tactaatggt aaacttactc ttaagtttat ttgcactact
      181 ggtaagttgc ctgttccttg gcctacttta gttactactc ttacttatgg tgttcaatgc
      241 ttttctcgtt atcctgatca tatgaagcaa catgattttt ttaagtctgc tatgcctgaa
      301 ggttatgttc aagaacgtac tatttctttt aaggatgatg gtacttacaa gactcgtgct
      361 gaagttaagt ttgaaggtga tactcttgtt aatcgtattg aacttaaggg tattgatttt
      421 aaagaagatg gtaatattct tggtcataag cttgaataca actttaattc tcataacgtt
      481 tacattactg ctgataagca aaagaacggt attaaggcta actttaagat tcgtcataat
      541 gttgaagatg gttctgttca acttgctgat cattaccaac aaaacactcc tattggtgat
      601 ggtcctgttc ttcttcctga taaccattac ctttctactc aatctgttct ttctaaggat
      661 cctaacgaaa agcgtgatca tatggttctt ttggaatttg ttactgctgc tggtattact
      721 cttggtatgg atgaacttta caagtaaaaa aagactaatg taaaattttt ttggttggtt
      781 attgaaaaag tcgatgcctt gtttgcgttt gttttcctag gcgttttatg tcagaaggca
      841 tttagaatta gtatacaagt actctttggt aaaattttat gtagcgacta aaatattaac
      901 tattatagat aaacaccttg ggaataaaaa gtaatttgct atagtaattt attaaacatg
      961 ctcctacaac attaccacaa tcttttctct tggattgaca ttgaataaga aaagagtgaa
     1021 tttttttaga cttgtaatga taactatgta caaagccaat gaaagatgta tgtagatgaa
     1081 tgtaaaatac catgtagaca aacaagataa aacttggtta taaacattgg tgttggaaca
     1141 gaataaatta gatgtcaaaa agtttcgtca atatcacggc gcgccagatc tgtttagctt
     1201 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     1261 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1321 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     1381 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     1441 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     1501 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     1561 gaacataaac aaccatgggt aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     1621 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     1681 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     1741 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     1801 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     1861 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     1921 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     1981 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     2041 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     2101 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     2161 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     2221 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     2281 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     2341 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     2401 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     2461 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     2521 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     2581 ctcgtccgag ggcaaaggaa taatcagtac tgacaataaa aagattcttg ttttcaagaa
     2641 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     2701 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     2761 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     2821 ctaacgccgc catccagttt aaac
//
