LOCUS       sequence-138281-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK293 (mAID-mCherry2-Hygro)
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag atctcgagca
       61 gctgaagctt accgctagca tggatagatc cggaaagcct gaactcaccg cgacgtctgt
      121 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
      181 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
      241 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
      301 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
      361 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
      421 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
      481 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
      541 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
      601 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
      661 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
      721 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
      781 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
      841 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
      901 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
      961 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     1021 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     1081 acgcccc
//
